Rapid assessment of CRISPR gRNAs with optimized protoplast transformation in Maize.

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Title: Rapid assessment of CRISPR gRNAs with optimized protoplast transformation in Maize.
Authors: Higa L; Department of Molecular Biosciences and Bioengineering, University of Hawai'i at Mānoa, Honolulu, HI, 96822, USA., Blank M; Department of Molecular Biosciences and Bioengineering, University of Hawai'i at Mānoa, Honolulu, HI, 96822, USA., Hampson E; Department of Tropical Plant and Soil Sciences, University of Hawai'i at Mānoa, Honolulu, HI, 96822, USA., Matsuyama J; Department of Molecular Biosciences and Bioengineering, University of Hawai'i at Mānoa, Honolulu, HI, 96822, USA., Wilkes K; Department of Molecular Biosciences and Bioengineering, University of Hawai'i at Mānoa, Honolulu, HI, 96822, USA., Uehara A; Department of Molecular Biosciences and Bioengineering, University of Hawai'i at Mānoa, Honolulu, HI, 96822, USA., Bouwman T; Department of Molecular Biosciences and Bioengineering, University of Hawai'i at Mānoa, Honolulu, HI, 96822, USA., Lee K; Department of Agronomy, Iowa State University, Ames, IA, USA.; Crop Bioengineering Center, Iowa State University, Ames, IA, USA., Wang K; Department of Agronomy, Iowa State University, Ames, IA, USA.; Crop Bioengineering Center, Iowa State University, Ames, IA, USA., Muszynski M; Department of Tropical Plant and Soil Sciences, University of Hawai'i at Mānoa, Honolulu, HI, 96822, USA., Du ZY; Department of Molecular Biosciences and Bioengineering, University of Hawai'i at Mānoa, Honolulu, HI, 96822, USA. duz@hawaii.edu.
Source: Plant cell reports [Plant Cell Rep] 2025 Sep 12; Vol. 44 (10), pp. 212. Date of Electronic Publication: 2025 Sep 12.
Publication Type: Journal Article
Journal Info: Publisher: Springer Country of Publication: Germany NLM ID: 9880970 Publication Model: Electronic Cited Medium: Internet ISSN: 1432-203X (Electronic) Linking ISSN: 07217714 NLM ISO Abbreviation: Plant Cell Rep Subsets: MEDLINE
Database: MEDLINE Ultimate
Description
ISSN:1432-203X
DOI:10.1007/s00299-025-03599-3