Evaluating urine volume and host depletion methods to enable genome-resolved metagenomics of the urobiome.

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Bibliographic Details
Title: Evaluating urine volume and host depletion methods to enable genome-resolved metagenomics of the urobiome.
Authors: Lewis ZJ; Department of Veterinary Preventive Medicine, The Ohio State University, Columbus, OH, USA.; Center of Microbiome Science, The Ohio State University, Columbus, OH, 43210, USA., Scott A; Department of Veterinary Preventive Medicine, The Ohio State University, Columbus, OH, USA., Madden C; Department of Veterinary Preventive Medicine, The Ohio State University, Columbus, OH, USA., Vik D; Center of Microbiome Science, The Ohio State University, Columbus, OH, 43210, USA.; Genomics and Microbiology Solutions Laboratory, Infectious Disease Institute, Columbus, OH, USA., Zayed AA; Department of Microbiology, The Ohio State University, Columbus, OH, USA.; EMERGE Biology Integration Institute, The Ohio State University, Columbus, OH, USA., Smith GJ; Center of Microbiome Science, The Ohio State University, Columbus, OH, 43210, USA.; Genomics and Microbiology Solutions Laboratory, Infectious Disease Institute, Columbus, OH, USA., Justice SS; College of Nursing, The Ohio State University, Columbus, OH, USA., Rudinsky A; Department of Veterinary Clinical Sciences, The Ohio State University, Columbus, OH, USA., Hokamp J; Department of Veterinary Biosciences, The Ohio State University, Columbus, OH, USA., Hale VL; Department of Veterinary Preventive Medicine, The Ohio State University, Columbus, OH, USA. Hale.502@osu.edu.; Center of Microbiome Science, The Ohio State University, Columbus, OH, 43210, USA. Hale.502@osu.edu.
Source: Microbiome [Microbiome] 2025 Oct 02; Vol. 13 (1), pp. 199. Date of Electronic Publication: 2025 Oct 02.
Publication Type: Journal Article; Research Support, Non-U.S. Gov't
Journal Info: Publisher: BioMed Central Country of Publication: England NLM ID: 101615147 Publication Model: Electronic Cited Medium: Internet ISSN: 2049-2618 (Electronic) Linking ISSN: 20492618 NLM ISO Abbreviation: Microbiome Subsets: MEDLINE
Database: MEDLINE Ultimate
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Description
ISSN:2049-2618
DOI:10.1186/s40168-025-02191-x