Evaluating urine volume and host depletion methods to enable genome-resolved metagenomics of the urobiome.

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Title: Evaluating urine volume and host depletion methods to enable genome-resolved metagenomics of the urobiome.
Authors: Lewis ZJ; Department of Veterinary Preventive Medicine, The Ohio State University, Columbus, OH, USA.; Center of Microbiome Science, The Ohio State University, Columbus, OH, 43210, USA., Scott A; Department of Veterinary Preventive Medicine, The Ohio State University, Columbus, OH, USA., Madden C; Department of Veterinary Preventive Medicine, The Ohio State University, Columbus, OH, USA., Vik D; Center of Microbiome Science, The Ohio State University, Columbus, OH, 43210, USA.; Genomics and Microbiology Solutions Laboratory, Infectious Disease Institute, Columbus, OH, USA., Zayed AA; Department of Microbiology, The Ohio State University, Columbus, OH, USA.; EMERGE Biology Integration Institute, The Ohio State University, Columbus, OH, USA., Smith GJ; Center of Microbiome Science, The Ohio State University, Columbus, OH, 43210, USA.; Genomics and Microbiology Solutions Laboratory, Infectious Disease Institute, Columbus, OH, USA., Justice SS; College of Nursing, The Ohio State University, Columbus, OH, USA., Rudinsky A; Department of Veterinary Clinical Sciences, The Ohio State University, Columbus, OH, USA., Hokamp J; Department of Veterinary Biosciences, The Ohio State University, Columbus, OH, USA., Hale VL; Department of Veterinary Preventive Medicine, The Ohio State University, Columbus, OH, USA. Hale.502@osu.edu.; Center of Microbiome Science, The Ohio State University, Columbus, OH, 43210, USA. Hale.502@osu.edu.
Source: Microbiome [Microbiome] 2025 Oct 02; Vol. 13 (1), pp. 199. Date of Electronic Publication: 2025 Oct 02.
Publication Type: Journal Article; Research Support, Non-U.S. Gov't
Journal Info: Publisher: BioMed Central Country of Publication: England NLM ID: 101615147 Publication Model: Electronic Cited Medium: Internet ISSN: 2049-2618 (Electronic) Linking ISSN: 20492618 NLM ISO Abbreviation: Microbiome Subsets: MEDLINE
Database: MEDLINE Ultimate
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  Data: <searchLink fieldCode="AU" term="%22Lewis+ZJ%22">Lewis ZJ</searchLink>; Department of Veterinary Preventive Medicine, The Ohio State University, Columbus, OH, USA.; Center of Microbiome Science, The Ohio State University, Columbus, OH, 43210, USA.<br /><searchLink fieldCode="AU" term="%22Scott+A%22">Scott A</searchLink>; Department of Veterinary Preventive Medicine, The Ohio State University, Columbus, OH, USA.<br /><searchLink fieldCode="AU" term="%22Madden+C%22">Madden C</searchLink>; Department of Veterinary Preventive Medicine, The Ohio State University, Columbus, OH, USA.<br /><searchLink fieldCode="AU" term="%22Vik+D%22">Vik D</searchLink>; Center of Microbiome Science, The Ohio State University, Columbus, OH, 43210, USA.; Genomics and Microbiology Solutions Laboratory, Infectious Disease Institute, Columbus, OH, USA.<br /><searchLink fieldCode="AU" term="%22Zayed+AA%22">Zayed AA</searchLink>; Department of Microbiology, The Ohio State University, Columbus, OH, USA.; EMERGE Biology Integration Institute, The Ohio State University, Columbus, OH, USA.<br /><searchLink fieldCode="AU" term="%22Smith+GJ%22">Smith GJ</searchLink>; Center of Microbiome Science, The Ohio State University, Columbus, OH, 43210, USA.; Genomics and Microbiology Solutions Laboratory, Infectious Disease Institute, Columbus, OH, USA.<br /><searchLink fieldCode="AU" term="%22Justice+SS%22">Justice SS</searchLink>; College of Nursing, The Ohio State University, Columbus, OH, USA.<br /><searchLink fieldCode="AU" term="%22Rudinsky+A%22">Rudinsky A</searchLink>; Department of Veterinary Clinical Sciences, The Ohio State University, Columbus, OH, USA.<br /><searchLink fieldCode="AU" term="%22Hokamp+J%22">Hokamp J</searchLink>; Department of Veterinary Biosciences, The Ohio State University, Columbus, OH, USA.<br /><searchLink fieldCode="AU" term="%22Hale+VL%22">Hale VL</searchLink>; Department of Veterinary Preventive Medicine, The Ohio State University, Columbus, OH, USA. Hale.502@osu.edu.; Center of Microbiome Science, The Ohio State University, Columbus, OH, 43210, USA. Hale.502@osu.edu.
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  Data: <i>Publisher: </i><searchLink fieldCode="PB" term="%22BioMed+Central%22">BioMed Central </searchLink><i>Country of Publication: </i>England <i>NLM ID: </i>101615147 <i>Publication Model: </i>Electronic <i>Cited Medium: </i>Internet <i>ISSN: </i>2049-2618 (Electronic) <i>Linking ISSN: </i><searchLink fieldCode="IS" term="%2220492618%22">20492618 </searchLink><i>NLM ISO Abbreviation: </i>Microbiome <i>Subsets: </i>MEDLINE
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              Text: 2025 Oct 02
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