Single-cell DNA methylation analysis tool Amethyst resolves distinct non-CG methylation patterns in human astrocytes and oligodendrocytes.

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Title: Single-cell DNA methylation analysis tool Amethyst resolves distinct non-CG methylation patterns in human astrocytes and oligodendrocytes.
Authors: Rylaarsdam LE; Department of Molecular & Medical Genetics, Oregon Health & Science University, Portland, OR, USA. rylaarsd@ohsu.edu., Skubi BW; Cancer Early Detection Advanced Research Center, Oregon Health & Science University, Portland, OR, USA.; Department of Biomedical Engineering, Oregon Health & Science University, Portland, OR, USA., Nichols RV; Department of Molecular & Medical Genetics, Oregon Health & Science University, Portland, OR, USA., O'Connell BL; Department of Molecular & Medical Genetics, Oregon Health & Science University, Portland, OR, USA.; Cancer Early Detection Advanced Research Center, Oregon Health & Science University, Portland, OR, USA., Kotnik JH; M.D./Ph.D. Training Program, Oregon Health & Science University, Portland, OR, USA., Coleman SD; Cancer Early Detection Advanced Research Center, Oregon Health & Science University, Portland, OR, USA.; Division of Oncological Sciences, Oregon Health & Science University, Portland, OR, USA.; Knight Cancer Institute, Oregon Health & Science University, Portland, OR, USA., Yardımcı GG; Cancer Early Detection Advanced Research Center, Oregon Health & Science University, Portland, OR, USA.; Division of Oncological Sciences, Oregon Health & Science University, Portland, OR, USA.; Knight Cancer Institute, Oregon Health & Science University, Portland, OR, USA., Adey AC; Department of Molecular & Medical Genetics, Oregon Health & Science University, Portland, OR, USA. adey@ohsu.edu.; Cancer Early Detection Advanced Research Center, Oregon Health & Science University, Portland, OR, USA. adey@ohsu.edu.; Knight Cancer Institute, Oregon Health & Science University, Portland, OR, USA. adey@ohsu.edu.; Knight Cardiovascular Institute, Oregon Health & Science University, Portland, OR, USA. adey@ohsu.edu.
Source: Communications biology [Commun Biol] 2025 Oct 14; Vol. 8 (1), pp. 1468. Date of Electronic Publication: 2025 Oct 14.
Publication Type: Journal Article
Journal Info: Publisher: Nature Publishing Group UK Country of Publication: England NLM ID: 101719179 Publication Model: Electronic Cited Medium: Internet ISSN: 2399-3642 (Electronic) Linking ISSN: 23993642 NLM ISO Abbreviation: Commun Biol Subsets: MEDLINE
Database: MEDLINE Ultimate
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ISSN:2399-3642
DOI:10.1038/s42003-025-08859-2