RECKLEEN is a lambda Red/CRISPR-Cas9 based single plasmid platform for enhanced genome editing in Klebsiella pneumoniae.

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Title: RECKLEEN is a lambda Red/CRISPR-Cas9 based single plasmid platform for enhanced genome editing in Klebsiella pneumoniae.
Authors: Elsayed EM; Center for Synthetic Microbiology (SYNMIKRO), Philipps-Universität Marburg, Marburg, Germany.; Department of Biology, Philipps-Universität Marburg, Marburg, Germany.; Department of Microbiology and Immunology, Faculty of Pharmacy, Zagazig University, Zagazig, Egypt., Stukenberg D; Center for Synthetic Microbiology (SYNMIKRO), Philipps-Universität Marburg, Marburg, Germany.; Department of Biology, Technical University Darmstadt, Darmstadt, Germany., Meier D; Center for Synthetic Microbiology (SYNMIKRO), Philipps-Universität Marburg, Marburg, Germany.; Department of Biology, Philipps-Universität Marburg, Marburg, Germany., Schmeck B; Center for Synthetic Microbiology (SYNMIKRO), Philipps-Universität Marburg, Marburg, Germany.; Institute for Lung Research, Universities of Giessen and Marburg Lung Center, German Center for Lung Research (DZL), Philipps-University Marburg, Marburg, Germany.; Department of Medicine, Pulmonary and Critical Care Medicine, University Medical Center Marburg, Universities of Giessen and Marburg Lung Center, Philipps-University Marburg, Marburg, Germany., Becker A; Center for Synthetic Microbiology (SYNMIKRO), Philipps-Universität Marburg, Marburg, Germany. anke.becker@synmikro.uni-marburg.de.; Department of Biology, Philipps-Universität Marburg, Marburg, Germany. anke.becker@synmikro.uni-marburg.de.
Source: Communications biology [Commun Biol] 2025 Oct 30; Vol. 8 (1), pp. 1509. Date of Electronic Publication: 2025 Oct 30.
Publication Type: Journal Article
Journal Info: Publisher: Nature Publishing Group UK Country of Publication: England NLM ID: 101719179 Publication Model: Electronic Cited Medium: Internet ISSN: 2399-3642 (Electronic) Linking ISSN: 23993642 NLM ISO Abbreviation: Commun Biol Subsets: MEDLINE
Database: MEDLINE Ultimate
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ISSN:2399-3642
DOI:10.1038/s42003-025-08934-8