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An optimized protocol for isolating Rhinosporidium seeberi sporangia from rhinosporidiosis tissue and extracting genomic DNA for next-generation sequencing.

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Bibliographic Details
Title: An optimized protocol for isolating Rhinosporidium seeberi sporangia from rhinosporidiosis tissue and extracting genomic DNA for next-generation sequencing.
Authors: Sasidharan SP; Multidisciplinary Research Unit (MRU), (Department of Health Research, Government of India), Government Medical College, Thiruvananthapuram, 695011, Kerala, India. sreejith2383@gmail.com.; Zebrafish Research Facility, (Multidisciplinary Research Unit, Department of Health Research, Government of India), Government Medical College, Thiruvananthapuram, 695011, Kerala, India. sreejith2383@gmail.com., James S; Department of ENT, Government Medical College, Thiruvananthapuram, 695011, Kerala, India., Balan S; The Center for Interdisciplinary Brain Sciences, Institute of Mental Health and Neurosciences (IMHANS), Kozhikode, 673008, Kerala, India., Manakkadan A; Multidisciplinary Research Unit (MRU), (Department of Health Research, Government of India), Government Medical College, Thiruvananthapuram, 695011, Kerala, India.
Source: Molecular biology reports [Mol Biol Rep] 2025 Nov 26; Vol. 53 (1), pp. 117. Date of Electronic Publication: 2025 Nov 26.
Publication Type: Journal Article
Journal Info: Publisher: Reidel Country of Publication: Netherlands NLM ID: 0403234 Publication Model: Electronic Cited Medium: Internet ISSN: 1573-4978 (Electronic) Linking ISSN: 03014851 NLM ISO Abbreviation: Mol Biol Rep Subsets: MEDLINE
Database: MEDLINE Ultimate
Description
ISSN:1573-4978
DOI:10.1007/s11033-025-11280-x