Chemically modified CRISPR-Cas9 enables targeting of individual G-quadruplex and i-motif structures, revealing ligand-dependent transcriptional perturbation.

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Title: Chemically modified CRISPR-Cas9 enables targeting of individual G-quadruplex and i-motif structures, revealing ligand-dependent transcriptional perturbation.
Authors: Nuccio SP; Imperial College London, Department of Chemistry, Molecular Sciences Research Hub, 82 Wood Lane, London, UK.; Imperial College London, Institute of Chemical Biology, Molecular Sciences Research Hub, 82 Wood Lane, London, UK., Cadoni E; Imperial College London, Department of Chemistry, Molecular Sciences Research Hub, 82 Wood Lane, London, UK.; Department of Organic and Macromolecular Chemistry - Organic and Biomimetic Chemistry Research Group (OBCR), Faculty of Sciences, Campus Sterre, Krijgslaan 281, Building S4, Gent, Belgium., Nikoloudaki R; Imperial College London, Department of Chemistry, Molecular Sciences Research Hub, 82 Wood Lane, London, UK., Galli S; Imperial College London, Department of Chemistry, Molecular Sciences Research Hub, 82 Wood Lane, London, UK., Ler AJ; Imperial College London, Department of Chemistry, Molecular Sciences Research Hub, 82 Wood Lane, London, UK., Sanchez-Cabanillas C; Imperial College London, Department of Chemistry, Molecular Sciences Research Hub, 82 Wood Lane, London, UK.; The Breast Cancer Now Toby Robins Research Centre, The Institute of Cancer Research, 123 Old Brompton Road, London, UK., Maher TE; Imperial College London, Department of Chemistry, Molecular Sciences Research Hub, 82 Wood Lane, London, UK.; Imperial College London, Institute of Chemical Biology, Molecular Sciences Research Hub, 82 Wood Lane, London, UK., Fan E; Imperial College London, Department of Chemistry, Molecular Sciences Research Hub, 82 Wood Lane, London, UK., Guneri D; UCL School of Pharmacy, 29-39 Brunswick Square, London, UK., Flint G; Imperial College London, Department of Chemistry, Molecular Sciences Research Hub, 82 Wood Lane, London, UK.; Imperial College London, Institute of Chemical Biology, Molecular Sciences Research Hub, 82 Wood Lane, London, UK., Zhu M; Imperial College London, Department of Chemistry, Molecular Sciences Research Hub, 82 Wood Lane, London, UK., Liu LS; Imperial College London, Department of Chemistry, Molecular Sciences Research Hub, 82 Wood Lane, London, UK., Fullenkamp CR; Chemical Biology Laboratory, National Cancer Institute, Frederick, MD, USA., Waller Z; UCL School of Pharmacy, 29-39 Brunswick Square, London, UK., Magnani L; The Breast Cancer Now Toby Robins Research Centre, The Institute of Cancer Research, 123 Old Brompton Road, London, UK.; Department of Oncology and Haemato-Oncology, Università degli Studi di Milano, Milan, Italy., Schneekloth JS Jr; Chemical Biology Laboratory, National Cancer Institute, Frederick, MD, USA., Di Antonio M; Imperial College London, Department of Chemistry, Molecular Sciences Research Hub, 82 Wood Lane, London, UK. m.di-antonio@imperial.ac.uk.; Imperial College London, Institute of Chemical Biology, Molecular Sciences Research Hub, 82 Wood Lane, London, UK. m.di-antonio@imperial.ac.uk.; The Francis Crick Institute, 1 Midland Road, London, UK. m.di-antonio@imperial.ac.uk.
Source: Nature communications [Nat Commun] 2025 Dec 09; Vol. 17 (1), pp. 385. Date of Electronic Publication: 2025 Dec 09.
Publication Type: Journal Article
Journal Info: Publisher: Nature Pub. Group Country of Publication: England NLM ID: 101528555 Publication Model: Electronic Cited Medium: Internet ISSN: 2041-1723 (Electronic) Linking ISSN: 20411723 NLM ISO Abbreviation: Nat Commun Subsets: MEDLINE
Database: MEDLINE Ultimate
Description
ISSN:2041-1723
DOI:10.1038/s41467-025-67074-z