Selecting the optimal cell migration assay: fundamentals and practical guidelines.

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Title: Selecting the optimal cell migration assay: fundamentals and practical guidelines.
Authors: Du W; Department of Chemical & Biomolecular Engineering, Johns Hopkins University, Baltimore, MD, USA.; Institute for NanoBioTechnology, Johns Hopkins University, Baltimore, MD, USA., Nair PR; Institute for NanoBioTechnology, Johns Hopkins University, Baltimore, MD, USA., Forjaz A; Department of Chemical & Biomolecular Engineering, Johns Hopkins University, Baltimore, MD, USA.; Institute for NanoBioTechnology, Johns Hopkins University, Baltimore, MD, USA., Phillip JM; Department of Chemical & Biomolecular Engineering, Johns Hopkins University, Baltimore, MD, USA. jphillip@jhu.edu.; Institute for NanoBioTechnology, Johns Hopkins University, Baltimore, MD, USA. jphillip@jhu.edu.; Department of Biomedical Engineering, Johns Hopkins University, Baltimore, MD, USA. jphillip@jhu.edu.; Translational Therapeutics and Regenerative Engineering Center, Johns Hopkins University, Baltimore, MD, USA. jphillip@jhu.edu.; Department of Oncology, Johns Hopkins School of Medicine, Johns Hopkins University, Baltimore, MD, USA. jphillip@jhu.edu., Wu PH; Department of Chemical & Biomolecular Engineering, Johns Hopkins University, Baltimore, MD, USA. pwu@jhu.edu.; Institute for NanoBioTechnology, Johns Hopkins University, Baltimore, MD, USA. pwu@jhu.edu., Wirtz D; Department of Chemical & Biomolecular Engineering, Johns Hopkins University, Baltimore, MD, USA. wirtz@jhu.edu.; Institute for NanoBioTechnology, Johns Hopkins University, Baltimore, MD, USA. wirtz@jhu.edu.; Department of Oncology, Johns Hopkins School of Medicine, Johns Hopkins University, Baltimore, MD, USA. wirtz@jhu.edu.; Department of Pathology, Johns Hopkins School of Medicine, Johns Hopkins University, Baltimore, MD, USA. wirtz@jhu.edu.
Source: Nature methods [Nat Methods] 2026 Jan; Vol. 23 (1), pp. 30-42. Date of Electronic Publication: 2025 Dec 18.
Publication Type: Journal Article; Review
Journal Info: Publisher: Nature Pub. Group Country of Publication: United States NLM ID: 101215604 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1548-7105 (Electronic) Linking ISSN: 15487091 NLM ISO Abbreviation: Nat Methods Subsets: MEDLINE
Database: MEDLINE Ultimate
Description
ISSN:1548-7105
DOI:10.1038/s41592-025-02890-1