Uneven sequencing (coverage) depth can bias microbial intraspecies diversity estimates and how to account for it.

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Title: Uneven sequencing (coverage) depth can bias microbial intraspecies diversity estimates and how to account for it.
Authors: Bustos-Caparros E; Marine Microbiology Group (MMG), Department of Animal and Microbial Biodiversity, Mediterranean Institute for Advanced Studies (IMEDEA, CSIC-UIB), 07190 Esporles, Spain., Viver T; Marine Microbiology Group (MMG), Department of Animal and Microbial Biodiversity, Mediterranean Institute for Advanced Studies (IMEDEA, CSIC-UIB), 07190 Esporles, Spain., Gago JF; Marine Microbiology Group (MMG), Department of Animal and Microbial Biodiversity, Mediterranean Institute for Advanced Studies (IMEDEA, CSIC-UIB), 07190 Esporles, Spain., Venter SN; Department of Biochemistry, Genetics and Microbiology, and Forestry and Agricultural Biotechnology Institute (FABI), University of Pretoria, 0002 Pretoria, South Africa., Bosch R; Microbiologia, Departament de Biologia, Edifici Guillem Colom, Universitat de les Illes Balears, Campus UIB, 07122 Palma de Mallorca, Spain., Konstantinidis KT; School of Civil and Environmental Engineering and School of Biological Sciences, Georgia Institute of Technology, 3033 Atlanta, Georgia, United States., Rodriguez-R LM; Department of Chemistry and Biosciences, Aalborg University, 9220 Aalborg, Denmark.; Department of Microbiology and Digital Science Center (DiSC), University of Innsbruck, 6020 Innsbruck, Austria., Rossello-Mora R; Marine Microbiology Group (MMG), Department of Animal and Microbial Biodiversity, Mediterranean Institute for Advanced Studies (IMEDEA, CSIC-UIB), 07190 Esporles, Spain.
Source: ISME communications [ISME Commun] 2025 Dec 06; Vol. 5 (1), pp. ycaf228. Date of Electronic Publication: 2025 Dec 06 (Print Publication: 2025).
Publication Type: Journal Article
Journal Info: Publisher: Oxford University Press Country of Publication: England NLM ID: 9918205372406676 Publication Model: eCollection Cited Medium: Internet ISSN: 2730-6151 (Electronic) Linking ISSN: 27306151 NLM ISO Abbreviation: ISME Commun Subsets: PubMed not MEDLINE
Database: MEDLINE Ultimate
Description
ISSN:2730-6151
DOI:10.1093/ismeco/ycaf228