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Hydrogen/deuterium exchange mass spectrometry analysis of ribosome-nascent chain complexes to study protein biogenesis at the peptide level.

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Title: Hydrogen/deuterium exchange mass spectrometry analysis of ribosome-nascent chain complexes to study protein biogenesis at the peptide level.
Authors: Roeselová A; Protein Biogenesis Laboratory, The Francis Crick Institute, London, UK., Pajak A; Protein Biogenesis Laboratory, The Francis Crick Institute, London, UK., Wales TE; Department of Chemistry and Chemical Biology, Northeastern University, Boston, MA, USA., Pellowe GA; Protein Biogenesis Laboratory, The Francis Crick Institute, London, UK.; Aston Institute for Membrane Excellence, Aston University, Birmingham, UK., Kjær S; Structural Biology Science Technology Platform, The Francis Crick Institute, London, UK., Engen JR; Department of Chemistry and Chemical Biology, Northeastern University, Boston, MA, USA. j.engen@northeastern.edu., Balchin D; Protein Biogenesis Laboratory, The Francis Crick Institute, London, UK. david.balchin@crick.ac.uk.
Source: Nature protocols [Nat Protoc] 2026 May; Vol. 21 (5), pp. 2271-2300. Date of Electronic Publication: 2026 Jan 12.
Publication Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, N.I.H., Intramural
Journal Info: Publisher: Nature Pub. Group Country of Publication: England NLM ID: 101284307 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1750-2799 (Electronic) Linking ISSN: 17502799 NLM ISO Abbreviation: Nat Protoc Subsets: MEDLINE
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  Data: Hydrogen/deuterium exchange mass spectrometry analysis of ribosome-nascent chain complexes to study protein biogenesis at the peptide level.
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  Data: <searchLink fieldCode="AU" term="%22Roeselová+A%22">Roeselová A</searchLink>; Protein Biogenesis Laboratory, The Francis Crick Institute, London, UK.<br /><searchLink fieldCode="AU" term="%22Pajak+A%22">Pajak A</searchLink>; Protein Biogenesis Laboratory, The Francis Crick Institute, London, UK.<br /><searchLink fieldCode="AU" term="%22Wales+TE%22">Wales TE</searchLink>; Department of Chemistry and Chemical Biology, Northeastern University, Boston, MA, USA.<br /><searchLink fieldCode="AU" term="%22Pellowe+GA%22">Pellowe GA</searchLink>; Protein Biogenesis Laboratory, The Francis Crick Institute, London, UK.; Aston Institute for Membrane Excellence, Aston University, Birmingham, UK.<br /><searchLink fieldCode="AU" term="%22Kjær+S%22">Kjær S</searchLink>; Structural Biology Science Technology Platform, The Francis Crick Institute, London, UK.<br /><searchLink fieldCode="AU" term="%22Engen+JR%22">Engen JR</searchLink>; Department of Chemistry and Chemical Biology, Northeastern University, Boston, MA, USA. j.engen@northeastern.edu.<br /><searchLink fieldCode="AU" term="%22Balchin+D%22">Balchin D</searchLink>; Protein Biogenesis Laboratory, The Francis Crick Institute, London, UK. david.balchin@crick.ac.uk.
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  Data: <searchLink fieldCode="JN" term="%22101284307%22">Nature protocols</searchLink> [Nat Protoc] 2026 May; Vol. 21 (5), pp. 2271-2300. <i>Date of Electronic Publication: </i>2026 Jan 12.
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        Value: 10.1038/s41596-025-01279-w
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              Text: 2026 May
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