Dual-TERM enables simultaneous profiling of 5' and 3' terminal modifications in small RNAs by integrating enzymatic treatment with PBA-PAGE.

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Title: Dual-TERM enables simultaneous profiling of 5' and 3' terminal modifications in small RNAs by integrating enzymatic treatment with PBA-PAGE.
Authors: Yang H; State Key Laboratory of Genetics and Development of Complex Phenotypes, Zhangjiang mRNA Innovation and Translation Center, School of Life Sciences, Fudan University, Shanghai, 200438, PR China., Zhang B; State Key Laboratory of Genetics and Development of Complex Phenotypes, Zhangjiang mRNA Innovation and Translation Center, School of Life Sciences, Fudan University, Shanghai, 200438, PR China., Zhu S; State Key Laboratory of Genetics and Development of Complex Phenotypes, Zhangjiang mRNA Innovation and Translation Center, School of Life Sciences, Fudan University, Shanghai, 200438, PR China., Zhang S; School of Pharmacy, Shanghai University of Traditional Chinese Medicine, Shanghai, 201203, PR China., Yu X; State Key Laboratory of Genetics and Development of Complex Phenotypes, Zhangjiang mRNA Innovation and Translation Center, School of Life Sciences, Fudan University, Shanghai, 200438, PR China., Zhang L; School of Pharmacy, Shanghai University of Traditional Chinese Medicine, Shanghai, 201203, PR China; Department of Pharmaceutical Botany, School of Pharmacy, Naval Medical University, Shanghai, 200438, PR China. Electronic address: zhanglei@smmu.edu.cn., Ren G; State Key Laboratory of Genetics and Development of Complex Phenotypes, Zhangjiang mRNA Innovation and Translation Center, School of Life Sciences, Fudan University, Shanghai, 200438, PR China. Electronic address: gdren@fudan.edu.cn., Chen S; School of Pharmacy, Shanghai University of Traditional Chinese Medicine, Shanghai, 201203, PR China; Department of Pharmaceutical Botany, School of Pharmacy, Naval Medical University, Shanghai, 200438, PR China; State Key Laboratory for Quality Ensurance and Sustainable Use of Dao-di Herbs, Beijing, 100700, PR China. Electronic address: sschen@shutcm.edu.cn.
Source: International journal of biological macromolecules [Int J Biol Macromol] 2026 Feb; Vol. 344 (Pt 1), pp. 150467. Date of Electronic Publication: 2026 Jan 23.
Publication Type: Journal Article
Journal Info: Publisher: Elsevier Country of Publication: Netherlands NLM ID: 7909578 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1879-0003 (Electronic) Linking ISSN: 01418130 NLM ISO Abbreviation: Int J Biol Macromol Subsets: MEDLINE
Database: MEDLINE Ultimate
Description
ISSN:1879-0003
DOI:10.1016/j.ijbiomac.2026.150467