Technical validation and implementation of a multiplex real-time PCR for differential diagnostic detection of Zika virus, yellow fever virus, dengue virus and chikungunya virus (ZYDC-PCR).
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| Title: | Technical validation and implementation of a multiplex real-time PCR for differential diagnostic detection of Zika virus, yellow fever virus, dengue virus and chikungunya virus (ZYDC-PCR). |
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| Authors: | Hauner A; Department of Biomedical Sciences, Unit of Virology, Institute of Tropical Medicine, Antwerp, Belgium. ahauner@itg.be., Benítez AJ; PAHO/WHO Collaborating Center for Dengue and its Control, Department of Virology, Pedro Kourí Institute of Tropical Medicine, Havana, Cuba., Mulopo-Mukanya N; Laboratoire Rodolphe Mérieux INRB-Goma, Goma, Democratic Republic of the Congo., Rogé S; DRC Office, Institute of Tropical Medicine, Antwerp, Belgium., Serrano S; PAHO/WHO Collaborating Center for Dengue and its Control, Department of Virology, Pedro Kourí Institute of Tropical Medicine, Havana, Cuba., Vanlerberghe V; Department of Public Health, Unit of Emerging Infectious Diseases, Institute of Tropical Medicine, Antwerp, Belgium., Lepore L; Department of Public Health, Unit of Emerging Infectious Diseases, Institute of Tropical Medicine, Antwerp, Belgium., Esbroeck MV; Department of Clinical Science, Institute of Tropical Medicine, Antwerp, Belgium., Masumu J; National Institute for Biomedical Research INRB, Kinshasa, Democratic Republic of the Congo., Nasimiyu C; Washington State University Global Health-Kenya, Nairobi, Kenya., Njenga MK; Center for Research in Emerging Infectious Diseases-East and Central Africa, Washington State University, Pullman, WA, USA., Romani MET; Department of Epidemiology, Pedro Kourí Institute of Tropical Medicine, Havana, Cuba., Portal YV; Laboratorio Provincial de Biología Molecular del Centro Provincial de Higiene Epidemiología y Microbiología, Cienfuegos, Cuba., Álvarez M; PAHO/WHO Collaborating Center for Dengue and its Control, Department of Virology, Pedro Kourí Institute of Tropical Medicine, Havana, Cuba., Ramírez-Bartutis R; PAHO/WHO Collaborating Center for Dengue and its Control, Department of Virology, Pedro Kourí Institute of Tropical Medicine, Havana, Cuba., Guzmán MG; PAHO/WHO Collaborating Center for Dengue and its Control, Department of Virology, Pedro Kourí Institute of Tropical Medicine, Havana, Cuba., Ahuka-Mundeke S; National Institute for Biomedical Research INRB, Kinshasa, Democratic Republic of the Congo., Mukadi-Bamuleka D; Laboratoire Rodolphe Mérieux INRB-Goma, Goma, Democratic Republic of the Congo.; National Institute for Biomedical Research INRB, Kinshasa, Democratic Republic of the Congo.; Service of Microbiology, Department of Medical Biology, Faculty of Medicine, University of Kinshasa, Kinshasa, Democratic Republic of the Congo., Ariën KK; Department of Biomedical Sciences, Unit of Virology, Institute of Tropical Medicine, Antwerp, Belgium.; Department of Biomedical Sciences, University of Antwerp, Antwerp, Belgium. |
| Source: | BMC infectious diseases [BMC Infect Dis] 2026 Feb 06; Vol. 26 (1). Date of Electronic Publication: 2026 Feb 06. |
| Publication Type: | Journal Article; Validation Study |
| Journal Info: | Publisher: BioMed Central Country of Publication: England NLM ID: 100968551 Publication Model: Electronic Cited Medium: Internet ISSN: 1471-2334 (Electronic) Linking ISSN: 14712334 NLM ISO Abbreviation: BMC Infect Dis Subsets: MEDLINE |
| Database: | MEDLINE Ultimate |
| FullText | Text: Availability: 0 |
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| Header | DbId: mdl DbLabel: MEDLINE Ultimate An: 41652539 AccessLevel: 2 PubType: Academic Journal PubTypeId: academicJournal PreciseRelevancyScore: 0 |
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| Items | – Name: Title Label: Title Group: Ti Data: Technical validation and implementation of a multiplex real-time PCR for differential diagnostic detection of Zika virus, yellow fever virus, dengue virus and chikungunya virus (ZYDC-PCR). – Name: Author Label: Authors Group: Au Data: <searchLink fieldCode="AU" term="%22Hauner+A%22">Hauner A</searchLink>; Department of Biomedical Sciences, Unit of Virology, Institute of Tropical Medicine, Antwerp, Belgium. ahauner@itg.be.<br /><searchLink fieldCode="AU" term="%22Benítez+AJ%22">Benítez AJ</searchLink>; PAHO/WHO Collaborating Center for Dengue and its Control, Department of Virology, Pedro Kourí Institute of Tropical Medicine, Havana, Cuba.<br /><searchLink fieldCode="AU" term="%22Mulopo-Mukanya+N%22">Mulopo-Mukanya N</searchLink>; Laboratoire Rodolphe Mérieux INRB-Goma, Goma, Democratic Republic of the Congo.<br /><searchLink fieldCode="AU" term="%22Rogé+S%22">Rogé S</searchLink>; DRC Office, Institute of Tropical Medicine, Antwerp, Belgium.<br /><searchLink fieldCode="AU" term="%22Serrano+S%22">Serrano S</searchLink>; PAHO/WHO Collaborating Center for Dengue and its Control, Department of Virology, Pedro Kourí Institute of Tropical Medicine, Havana, Cuba.<br /><searchLink fieldCode="AU" term="%22Vanlerberghe+V%22">Vanlerberghe V</searchLink>; Department of Public Health, Unit of Emerging Infectious Diseases, Institute of Tropical Medicine, Antwerp, Belgium.<br /><searchLink fieldCode="AU" term="%22Lepore+L%22">Lepore L</searchLink>; Department of Public Health, Unit of Emerging Infectious Diseases, Institute of Tropical Medicine, Antwerp, Belgium.<br /><searchLink fieldCode="AU" term="%22Esbroeck+MV%22">Esbroeck MV</searchLink>; Department of Clinical Science, Institute of Tropical Medicine, Antwerp, Belgium.<br /><searchLink fieldCode="AU" term="%22Masumu+J%22">Masumu J</searchLink>; National Institute for Biomedical Research INRB, Kinshasa, Democratic Republic of the Congo.<br /><searchLink fieldCode="AU" term="%22Nasimiyu+C%22">Nasimiyu C</searchLink>; Washington State University Global Health-Kenya, Nairobi, Kenya.<br /><searchLink fieldCode="AU" term="%22Njenga+MK%22">Njenga MK</searchLink>; Center for Research in Emerging Infectious Diseases-East and Central Africa, Washington State University, Pullman, WA, USA.<br /><searchLink fieldCode="AU" term="%22Romani+MET%22">Romani MET</searchLink>; Department of Epidemiology, Pedro Kourí Institute of Tropical Medicine, Havana, Cuba.<br /><searchLink fieldCode="AU" term="%22Portal+YV%22">Portal YV</searchLink>; Laboratorio Provincial de Biología Molecular del Centro Provincial de Higiene Epidemiología y Microbiología, Cienfuegos, Cuba.<br /><searchLink fieldCode="AU" term="%22Álvarez+M%22">Álvarez M</searchLink>; PAHO/WHO Collaborating Center for Dengue and its Control, Department of Virology, Pedro Kourí Institute of Tropical Medicine, Havana, Cuba.<br /><searchLink fieldCode="AU" term="%22Ramírez-Bartutis+R%22">Ramírez-Bartutis R</searchLink>; PAHO/WHO Collaborating Center for Dengue and its Control, Department of Virology, Pedro Kourí Institute of Tropical Medicine, Havana, Cuba.<br /><searchLink fieldCode="AU" term="%22Guzmán+MG%22">Guzmán MG</searchLink>; PAHO/WHO Collaborating Center for Dengue and its Control, Department of Virology, Pedro Kourí Institute of Tropical Medicine, Havana, Cuba.<br /><searchLink fieldCode="AU" term="%22Ahuka-Mundeke+S%22">Ahuka-Mundeke S</searchLink>; National Institute for Biomedical Research INRB, Kinshasa, Democratic Republic of the Congo.<br /><searchLink fieldCode="AU" term="%22Mukadi-Bamuleka+D%22">Mukadi-Bamuleka D</searchLink>; Laboratoire Rodolphe Mérieux INRB-Goma, Goma, Democratic Republic of the Congo.; National Institute for Biomedical Research INRB, Kinshasa, Democratic Republic of the Congo.; Service of Microbiology, Department of Medical Biology, Faculty of Medicine, University of Kinshasa, Kinshasa, Democratic Republic of the Congo.<br /><searchLink fieldCode="AU" term="%22Ariën+KK%22">Ariën KK</searchLink>; Department of Biomedical Sciences, Unit of Virology, Institute of Tropical Medicine, Antwerp, Belgium.; Department of Biomedical Sciences, University of Antwerp, Antwerp, Belgium. – Name: TitleSource Label: Source Group: Src Data: <searchLink fieldCode="JN" term="%22100968551%22">BMC infectious diseases</searchLink> [BMC Infect Dis] 2026 Feb 06; Vol. 26 (1). <i>Date of Electronic Publication: </i>2026 Feb 06. – Name: TypePub Label: Publication Type Group: TypPub Data: Journal Article; Validation Study – Name: TitleSource Label: Journal Info Group: Src Data: <i>Publisher: </i><searchLink fieldCode="PB" term="%22BioMed+Central%22">BioMed Central </searchLink><i>Country of Publication: </i>England <i>NLM ID: </i>100968551 <i>Publication Model: </i>Electronic <i>Cited Medium: </i>Internet <i>ISSN: </i>1471-2334 (Electronic) <i>Linking ISSN: </i><searchLink fieldCode="IS" term="%2214712334%22">14712334 </searchLink><i>NLM ISO Abbreviation: </i>BMC Infect Dis <i>Subsets: </i>MEDLINE |
| PLink | https://search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=mdl&AN=41652539 |
| RecordInfo | BibRecord: BibEntity: Identifiers: – Type: doi Value: 10.1186/s12879-026-12570-w Languages: – Code: eng Text: English Titles: – TitleFull: Technical validation and implementation of a multiplex real-time PCR for differential diagnostic detection of Zika virus, yellow fever virus, dengue virus and chikungunya virus (ZYDC-PCR). Type: main BibRelationships: HasContributorRelationships: – PersonEntity: Name: NameFull: Hauner A – PersonEntity: Name: NameFull: Benítez AJ – PersonEntity: Name: NameFull: Mulopo-Mukanya N – PersonEntity: Name: NameFull: Rogé S – PersonEntity: Name: NameFull: Serrano S – PersonEntity: Name: NameFull: Vanlerberghe V – PersonEntity: Name: NameFull: Lepore L – PersonEntity: Name: NameFull: Esbroeck MV – PersonEntity: Name: NameFull: Masumu J – PersonEntity: Name: NameFull: Nasimiyu C – PersonEntity: Name: NameFull: Njenga MK – PersonEntity: Name: NameFull: Romani MET – PersonEntity: Name: NameFull: Portal YV – PersonEntity: Name: NameFull: Álvarez M – PersonEntity: Name: NameFull: Ramírez-Bartutis R – PersonEntity: Name: NameFull: Guzmán MG – PersonEntity: Name: NameFull: Ahuka-Mundeke S – PersonEntity: Name: NameFull: Mukadi-Bamuleka D – PersonEntity: Name: NameFull: Ariën KK IsPartOfRelationships: – BibEntity: Dates: – D: 06 M: 02 Text: 2026 Feb 06 Type: published Y: 2026 Identifiers: – Type: issn-electronic Value: 1471-2334 Numbering: – Type: volume Value: 26 – Type: issue Value: 1 Titles: – TitleFull: BMC infectious diseases Type: main |
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