Technical validation and implementation of a multiplex real-time PCR for differential diagnostic detection of Zika virus, yellow fever virus, dengue virus and chikungunya virus (ZYDC-PCR).

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Title: Technical validation and implementation of a multiplex real-time PCR for differential diagnostic detection of Zika virus, yellow fever virus, dengue virus and chikungunya virus (ZYDC-PCR).
Authors: Hauner A; Department of Biomedical Sciences, Unit of Virology, Institute of Tropical Medicine, Antwerp, Belgium. ahauner@itg.be., Benítez AJ; PAHO/WHO Collaborating Center for Dengue and its Control, Department of Virology, Pedro Kourí Institute of Tropical Medicine, Havana, Cuba., Mulopo-Mukanya N; Laboratoire Rodolphe Mérieux INRB-Goma, Goma, Democratic Republic of the Congo., Rogé S; DRC Office, Institute of Tropical Medicine, Antwerp, Belgium., Serrano S; PAHO/WHO Collaborating Center for Dengue and its Control, Department of Virology, Pedro Kourí Institute of Tropical Medicine, Havana, Cuba., Vanlerberghe V; Department of Public Health, Unit of Emerging Infectious Diseases, Institute of Tropical Medicine, Antwerp, Belgium., Lepore L; Department of Public Health, Unit of Emerging Infectious Diseases, Institute of Tropical Medicine, Antwerp, Belgium., Esbroeck MV; Department of Clinical Science, Institute of Tropical Medicine, Antwerp, Belgium., Masumu J; National Institute for Biomedical Research INRB, Kinshasa, Democratic Republic of the Congo., Nasimiyu C; Washington State University Global Health-Kenya, Nairobi, Kenya., Njenga MK; Center for Research in Emerging Infectious Diseases-East and Central Africa, Washington State University, Pullman, WA, USA., Romani MET; Department of Epidemiology, Pedro Kourí Institute of Tropical Medicine, Havana, Cuba., Portal YV; Laboratorio Provincial de Biología Molecular del Centro Provincial de Higiene Epidemiología y Microbiología, Cienfuegos, Cuba., Álvarez M; PAHO/WHO Collaborating Center for Dengue and its Control, Department of Virology, Pedro Kourí Institute of Tropical Medicine, Havana, Cuba., Ramírez-Bartutis R; PAHO/WHO Collaborating Center for Dengue and its Control, Department of Virology, Pedro Kourí Institute of Tropical Medicine, Havana, Cuba., Guzmán MG; PAHO/WHO Collaborating Center for Dengue and its Control, Department of Virology, Pedro Kourí Institute of Tropical Medicine, Havana, Cuba., Ahuka-Mundeke S; National Institute for Biomedical Research INRB, Kinshasa, Democratic Republic of the Congo., Mukadi-Bamuleka D; Laboratoire Rodolphe Mérieux INRB-Goma, Goma, Democratic Republic of the Congo.; National Institute for Biomedical Research INRB, Kinshasa, Democratic Republic of the Congo.; Service of Microbiology, Department of Medical Biology, Faculty of Medicine, University of Kinshasa, Kinshasa, Democratic Republic of the Congo., Ariën KK; Department of Biomedical Sciences, Unit of Virology, Institute of Tropical Medicine, Antwerp, Belgium.; Department of Biomedical Sciences, University of Antwerp, Antwerp, Belgium.
Source: BMC infectious diseases [BMC Infect Dis] 2026 Feb 06; Vol. 26 (1). Date of Electronic Publication: 2026 Feb 06.
Publication Type: Journal Article; Validation Study
Journal Info: Publisher: BioMed Central Country of Publication: England NLM ID: 100968551 Publication Model: Electronic Cited Medium: Internet ISSN: 1471-2334 (Electronic) Linking ISSN: 14712334 NLM ISO Abbreviation: BMC Infect Dis Subsets: MEDLINE
Database: MEDLINE Ultimate
Description
ISSN:1471-2334
DOI:10.1186/s12879-026-12570-w