Heterogeneity Primer Spacers Improve the Performance of Massively Parallel Amplicon Sequencing of the V3-V4 Region of the 16 S rDNA as well as the 18 S Region for Blastocystis Subtyping.

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Title: Heterogeneity Primer Spacers Improve the Performance of Massively Parallel Amplicon Sequencing of the V3-V4 Region of the 16 S rDNA as well as the 18 S Region for Blastocystis Subtyping.
Authors: Cinek O; Department of Medical Microbiology, Second Faculty of Medicine, Charles University, University Hospital Motol, V Úvalu 84, Prague 5, Prague, Czechia, CZ-15006, Czechia. ondrej.cinek@Lfmotol.cuni.cz., Hubáčková K; Department of Medical Microbiology, Second Faculty of Medicine, Charles University, University Hospital Motol, V Úvalu 84, Prague 5, Prague, Czechia, CZ-15006, Czechia., Litošová K; Department of Medical Microbiology, Second Faculty of Medicine, Charles University, University Hospital Motol, V Úvalu 84, Prague 5, Prague, Czechia, CZ-15006, Czechia., Hlináková L; Department of Medical Microbiology, Second Faculty of Medicine, Charles University, University Hospital Motol, V Úvalu 84, Prague 5, Prague, Czechia, CZ-15006, Czechia.
Source: Microbial ecology [Microb Ecol] 2026 Mar 04; Vol. 89 (1). Date of Electronic Publication: 2026 Mar 04.
Publication Type: Journal Article
Journal Info: Publisher: Springer-Verlag Country of Publication: United States NLM ID: 7500663 Publication Model: Electronic Cited Medium: Internet ISSN: 1432-184X (Electronic) Linking ISSN: 00953628 NLM ISO Abbreviation: Microb Ecol Subsets: MEDLINE
Database: MEDLINE Ultimate
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ISSN:1432-184X
DOI:10.1007/s00248-026-02708-3