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Optimizing CRISPR precision in mouse embryos via microhomology-mediated end joining-dominant targeting.

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Title: Optimizing CRISPR precision in mouse embryos via microhomology-mediated end joining-dominant targeting.
Authors: Lkhagvadorj K; Department of Stem Cells and Human Disease Models, Research Center for Animal Life Science, Shiga University of Medical Science, Otsu, Shiga, Japan., Okamura E; Department of Stem Cells and Human Disease Models, Research Center for Animal Life Science, Shiga University of Medical Science, Otsu, Shiga, Japan. eiokamu@belle.shiga-med.ac.jp., Taki T; Ph.D. Program in Human Biology, School of Integrative and Global Majors, University of Tsukuba, Tsukuba, Ibaraki, Japan., Suzuki H; Laboratory Animal Resource Center in Transborder Medical Research Center, University of Tsukuba, Tsukuba, Ibaraki, Japan., Kuno A; Laboratory Animal Resource Center in Transborder Medical Research Center, University of Tsukuba, Tsukuba, Ibaraki, Japan.; Department of Anatomy and Embryology, Institute of Medicine, University of Tsukuba, Tsukuba, Ibaraki, Japan.; Tsukuba Institute for Advanced Research (TIAR), University of Tsukuba, Tsukuba, Ibaraki, Japan., Itoh Y; Division of Pathogenesis and Disease Regulation, Department of Pathology and Central Research Laboratory, Shiga University of Medical Science, Otsu, Shiga, Japan., Mizuno S; Laboratory Animal Resource Center in Transborder Medical Research Center, University of Tsukuba, Tsukuba, Ibaraki, Japan. konezumi@md.tsukuba.ac.jp., Woltjen K; Department of Life Science Frontiers, Center for iPS Cell Research and Application (CiRA), Kyoto University, Sakyo-ku, Kyoto, Japan. woltjen@cira.kyoto-u.ac.jp., Ema M; Department of Stem Cells and Human Disease Models, Research Center for Animal Life Science, Shiga University of Medical Science, Otsu, Shiga, Japan. mema@belle.shiga-med.ac.jp.; Institute for the Advanced Study of Human Biology (ASHBi), Kyoto University, Yoshida-Konoe-cho, Sakyo-ku, Kyoto, Japan. mema@belle.shiga-med.ac.jp.
Source: Communications biology [Commun Biol] 2026 Mar 23; Vol. 9 (1). Date of Electronic Publication: 2026 Mar 23.
Publication Type: Journal Article
Journal Info: Publisher: Nature Publishing Group UK Country of Publication: England NLM ID: 101719179 Publication Model: Electronic Cited Medium: Internet ISSN: 2399-3642 (Electronic) Linking ISSN: 23993642 NLM ISO Abbreviation: Commun Biol Subsets: MEDLINE
Database: MEDLINE Ultimate
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  Data: Optimizing CRISPR precision in mouse embryos via microhomology-mediated end joining-dominant targeting.
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  Data: <searchLink fieldCode="AU" term="%22Lkhagvadorj+K%22">Lkhagvadorj K</searchLink>; Department of Stem Cells and Human Disease Models, Research Center for Animal Life Science, Shiga University of Medical Science, Otsu, Shiga, Japan.<br /><searchLink fieldCode="AU" term="%22Okamura+E%22">Okamura E</searchLink>; Department of Stem Cells and Human Disease Models, Research Center for Animal Life Science, Shiga University of Medical Science, Otsu, Shiga, Japan. eiokamu@belle.shiga-med.ac.jp.<br /><searchLink fieldCode="AU" term="%22Taki+T%22">Taki T</searchLink>; Ph.D. Program in Human Biology, School of Integrative and Global Majors, University of Tsukuba, Tsukuba, Ibaraki, Japan.<br /><searchLink fieldCode="AU" term="%22Suzuki+H%22">Suzuki H</searchLink>; Laboratory Animal Resource Center in Transborder Medical Research Center, University of Tsukuba, Tsukuba, Ibaraki, Japan.<br /><searchLink fieldCode="AU" term="%22Kuno+A%22">Kuno A</searchLink>; Laboratory Animal Resource Center in Transborder Medical Research Center, University of Tsukuba, Tsukuba, Ibaraki, Japan.; Department of Anatomy and Embryology, Institute of Medicine, University of Tsukuba, Tsukuba, Ibaraki, Japan.; Tsukuba Institute for Advanced Research (TIAR), University of Tsukuba, Tsukuba, Ibaraki, Japan.<br /><searchLink fieldCode="AU" term="%22Itoh+Y%22">Itoh Y</searchLink>; Division of Pathogenesis and Disease Regulation, Department of Pathology and Central Research Laboratory, Shiga University of Medical Science, Otsu, Shiga, Japan.<br /><searchLink fieldCode="AU" term="%22Mizuno+S%22">Mizuno S</searchLink>; Laboratory Animal Resource Center in Transborder Medical Research Center, University of Tsukuba, Tsukuba, Ibaraki, Japan. konezumi@md.tsukuba.ac.jp.<br /><searchLink fieldCode="AU" term="%22Woltjen+K%22">Woltjen K</searchLink>; Department of Life Science Frontiers, Center for iPS Cell Research and Application (CiRA), Kyoto University, Sakyo-ku, Kyoto, Japan. woltjen@cira.kyoto-u.ac.jp.<br /><searchLink fieldCode="AU" term="%22Ema+M%22">Ema M</searchLink>; Department of Stem Cells and Human Disease Models, Research Center for Animal Life Science, Shiga University of Medical Science, Otsu, Shiga, Japan. mema@belle.shiga-med.ac.jp.; Institute for the Advanced Study of Human Biology (ASHBi), Kyoto University, Yoshida-Konoe-cho, Sakyo-ku, Kyoto, Japan. mema@belle.shiga-med.ac.jp.
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  Data: <searchLink fieldCode="JN" term="%22101719179%22">Communications biology</searchLink> [Commun Biol] 2026 Mar 23; Vol. 9 (1). <i>Date of Electronic Publication: </i>2026 Mar 23.
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  Data: <i>Publisher: </i><searchLink fieldCode="PB" term="%22Nature+Publishing+Group+UK%22">Nature Publishing Group UK </searchLink><i>Country of Publication: </i>England <i>NLM ID: </i>101719179 <i>Publication Model: </i>Electronic <i>Cited Medium: </i>Internet <i>ISSN: </i>2399-3642 (Electronic) <i>Linking ISSN: </i><searchLink fieldCode="IS" term="%2223993642%22">23993642 </searchLink><i>NLM ISO Abbreviation: </i>Commun Biol <i>Subsets: </i>MEDLINE
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              Text: 2026 Mar 23
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