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The 12 o'clock assay: an optimized dodecaplex droplet digital PCR assay for robust DNA methylation quantification and epigenetic clock-based age-predictions.

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Title: The 12 o'clock assay: an optimized dodecaplex droplet digital PCR assay for robust DNA methylation quantification and epigenetic clock-based age-predictions.
Authors: Hchaichi I; Laboratory for Genomics, Foundation Jean Dausset - CEPH, 75010, Paris, France., Garali I; PSE-SANTE/SERAMED/LRAcc, Autorité de Sûreté Nucléaire et de Radioprotection (ASNR), 92260, Fontenay-Aux-Roses, France., Popa AM; Laboratory for Genomics, Foundation Jean Dausset - CEPH, 75010, Paris, France., Daunay A; Laboratory for Genomics, Foundation Jean Dausset - CEPH, 75010, Paris, France., Tessier NP; Laboratory for Genomics, Foundation Jean Dausset - CEPH, 75010, Paris, France., Sahbatou M; Laboratory for Bioinformatics, Foundation Jean Dausset - CEPH, Paris, France., Hardy LM; Laboratory for Genomics, Foundation Jean Dausset - CEPH, 75010, Paris, France., Rampanou A; Circulating Tumor Biomarkers Laboratory, Inserm CIC-BT 1428, Department of Translational Research, Institut Curie, Paris, France., Blanché H; CEPH-Biobank, Foundation Jean Dausset - CEPH, Paris, France., Zagury JF; Équipe Génomique, Bioinformatique et Chimie Moléculaire (EA 7528), Conservatoire National des Arts et Métiers, Paris, France., Touvier M; INSERM, INRAE, CNAM, Centre for Research in Epidemiology and StatisticS (CRESS), Nutritional Epidemiology Research Team (EREN), Université Sorbonne Paris Nord and Université Paris Cité, 93017, Bobigny, France., Le Buanec H; INSERM U976 - HIPI Unit, Saint-Louis Research Institute, University of Paris, Paris, France., Renault S; Circulating Tumor Biomarkers Laboratory, Inserm CIC-BT 1428, Department of Translational Research, Institut Curie, Paris, France., Girerd N; Centre d'Investigation Clinique Plurithématique 1433 and Inserm U1116, CHRU Nancy, F-CRIN INI-CRCT (Cardiovascular and Renal Clinical Trialists), Université de Lorraine, Nancy, France., Deleuze JF; Laboratory for Genomics, Foundation Jean Dausset - CEPH, 75010, Paris, France.; Laboratory for Bioinformatics, Foundation Jean Dausset - CEPH, Paris, France.; CEPH-Biobank, Foundation Jean Dausset - CEPH, Paris, France.; Centre National de Recherche en Génomique Humaine, CEA, Institut François Jacob, Evry, France., How-Kit A; Laboratory for Genomics, Foundation Jean Dausset - CEPH, 75010, Paris, France. ahowkit@cephb.fr.
Source: Clinical epigenetics [Clin Epigenetics] 2026 Mar 24; Vol. 18 (1). Date of Electronic Publication: 2026 Mar 24.
Publication Type: Journal Article
Journal Info: Publisher: Biomed Central Country of Publication: Germany NLM ID: 101516977 Publication Model: Electronic Cited Medium: Internet ISSN: 1868-7083 (Electronic) Linking ISSN: 18687075 NLM ISO Abbreviation: Clin Epigenetics Subsets: MEDLINE
Database: MEDLINE Ultimate
Description
ISSN:1868-7083
DOI:10.1186/s13148-026-02105-0