Affinity enhancement of an anti-VEGF humanized nanobody as a superior candidate to bevacizumab: integrative in silico and in vitro studies.

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Title: Affinity enhancement of an anti-VEGF humanized nanobody as a superior candidate to bevacizumab: integrative in silico and in vitro studies.
Authors: Kazemi MS; Biotechnology Research Center, Venom and Biotherapeutics Molecules Laboratory, Pasteur Institute of Iran, Tehran, Iran.; Student Research Committee, Pasteur Institute of Iran, Tehran, Iran., Arab SS; Center of Molecular Medicine, Georgia University, Athens, GA, 30602, USA., Kazemi-Lomedasht F; Biotechnology Research Center, Venom and Biotherapeutics Molecules Laboratory, Pasteur Institute of Iran, Tehran, Iran., Ahangari Cohan R; Nanobiotechnology Department, New Technologies Research Group, Pasteur Institute of Iran, Tehran, Iran., Zandi F; Biotechnology Research Center, Pasteur Institute of Iran, Tehran, Iran., Shoari A; Department of Cancer Biology, Mayo Clinic, Jacksonville, FL, USA., Hosseininejad-Chafi M; Biotechnology Research Center, Venom and Biotherapeutics Molecules Laboratory, Pasteur Institute of Iran, Tehran, Iran., Mahjoobi G; Biotechnology Research Center, Venom and Biotherapeutics Molecules Laboratory, Pasteur Institute of Iran, Tehran, Iran., Ashja Ardalan A; Department of Pharmacy and Biotechnology, University of Bologna, 40126, Bologna, Italy., Kanavi MR; Ocular Tissue Engineering Research Center, Research Institute for Ophthalmology and Vision Science, Shahid Beheshti University of Medical Sciences, Tehran, Iran. rezaeikanavi@gmail.com., Behdani M; Biotechnology Research Center, Venom and Biotherapeutics Molecules Laboratory, Pasteur Institute of Iran, Tehran, Iran. behdani@pasteur.ac.ir.
Source: BMC biotechnology [BMC Biotechnol] 2026 May 12; Vol. 26 (1). Date of Electronic Publication: 2026 May 12.
Publication Type: Journal Article
Journal Info: Publisher: BioMed Central Country of Publication: England NLM ID: 101088663 Publication Model: Electronic Cited Medium: Internet ISSN: 1472-6750 (Electronic) Linking ISSN: 14726750 NLM ISO Abbreviation: BMC Biotechnol Subsets: MEDLINE
Database: MEDLINE Ultimate
Description
ISSN:1472-6750
DOI:10.1186/s12896-026-01165-7