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Cas9+ conditionally immortalized neutrophil progenitors as a tool for genome-wide CRISPR screening for neutrophil differentiation and function.

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Title: Cas9+ conditionally immortalized neutrophil progenitors as a tool for genome-wide CRISPR screening for neutrophil differentiation and function.
Authors: Jong RM; Department of Molecular and Cell Biology, Division of Immunology and Pathogenesis, University of California, Berkeley, Berkeley, United States.; School of Public Health, Division of Infectious Diseases and Vaccinology, University of California, Berkeley, Berkeley, United States., Ching KL; Department of Molecular and Cell Biology, Division of Immunology and Pathogenesis, University of California, Berkeley, Berkeley, United States., Garelis NE; Department of Molecular and Cell Biology, Division of Immunology and Pathogenesis, University of California, Berkeley, Berkeley, United States., Zilinskas A; Department of Molecular and Cell Biology, Division of Immunology and Pathogenesis, University of California, Berkeley, Berkeley, United States., Wrynla XH; Department of Molecular and Cell Biology, Division of Immunology and Pathogenesis, University of California, Berkeley, Berkeley, United States.; School of Public Health, Division of Infectious Diseases and Vaccinology, University of California, Berkeley, Berkeley, United States., Rawal S; Department of Molecular and Cell Biology, Division of Immunology and Pathogenesis, University of California, Berkeley, Berkeley, United States., Hill BC; Department of Molecular and Cell Biology, Division of Immunology and Pathogenesis, University of California, Berkeley, Berkeley, United States., Luckie BA; Department of Molecular and Cell Biology, Division of Immunology and Pathogenesis, University of California, Berkeley, Berkeley, United States., Shallow L; Department of Molecular and Cell Biology, Division of Immunology and Pathogenesis, University of California, Berkeley, Berkeley, United States., Cox JS; Department of Molecular and Cell Biology, Division of Immunology and Pathogenesis, University of California, Berkeley, Berkeley, United States., Barton GM; Department of Molecular and Cell Biology, Division of Immunology and Pathogenesis, University of California, Berkeley, Berkeley, United States., Stanley SA; Department of Molecular and Cell Biology, Division of Immunology and Pathogenesis, University of California, Berkeley, Berkeley, United States.; School of Public Health, Division of Infectious Diseases and Vaccinology, University of California, Berkeley, Berkeley, United States.
Source: ELife [Elife] 2026 May 18; Vol. 15. Date of Electronic Publication: 2026 May 18.
Publication Type: Journal Article
Journal Info: Publisher: eLife Sciences Publications, Ltd Country of Publication: England NLM ID: 101579614 Publication Model: Electronic Cited Medium: Internet ISSN: 2050-084X (Electronic) Linking ISSN: 2050084X NLM ISO Abbreviation: Elife Subsets: MEDLINE
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  Data: Cas9<superscript>+</superscript> conditionally immortalized neutrophil progenitors as a tool for genome-wide CRISPR screening for neutrophil differentiation and function.
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  Data: <searchLink fieldCode="AU" term="%22Jong+RM%22">Jong RM</searchLink>; Department of Molecular and Cell Biology, Division of Immunology and Pathogenesis, University of California, Berkeley, Berkeley, United States.; School of Public Health, Division of Infectious Diseases and Vaccinology, University of California, Berkeley, Berkeley, United States.<br /><searchLink fieldCode="AU" term="%22Ching+KL%22">Ching KL</searchLink>; Department of Molecular and Cell Biology, Division of Immunology and Pathogenesis, University of California, Berkeley, Berkeley, United States.<br /><searchLink fieldCode="AU" term="%22Garelis+NE%22">Garelis NE</searchLink>; Department of Molecular and Cell Biology, Division of Immunology and Pathogenesis, University of California, Berkeley, Berkeley, United States.<br /><searchLink fieldCode="AU" term="%22Zilinskas+A%22">Zilinskas A</searchLink>; Department of Molecular and Cell Biology, Division of Immunology and Pathogenesis, University of California, Berkeley, Berkeley, United States.<br /><searchLink fieldCode="AU" term="%22Wrynla+XH%22">Wrynla XH</searchLink>; Department of Molecular and Cell Biology, Division of Immunology and Pathogenesis, University of California, Berkeley, Berkeley, United States.; School of Public Health, Division of Infectious Diseases and Vaccinology, University of California, Berkeley, Berkeley, United States.<br /><searchLink fieldCode="AU" term="%22Rawal+S%22">Rawal S</searchLink>; Department of Molecular and Cell Biology, Division of Immunology and Pathogenesis, University of California, Berkeley, Berkeley, United States.<br /><searchLink fieldCode="AU" term="%22Hill+BC%22">Hill BC</searchLink>; Department of Molecular and Cell Biology, Division of Immunology and Pathogenesis, University of California, Berkeley, Berkeley, United States.<br /><searchLink fieldCode="AU" term="%22Luckie+BA%22">Luckie BA</searchLink>; Department of Molecular and Cell Biology, Division of Immunology and Pathogenesis, University of California, Berkeley, Berkeley, United States.<br /><searchLink fieldCode="AU" term="%22Shallow+L%22">Shallow L</searchLink>; Department of Molecular and Cell Biology, Division of Immunology and Pathogenesis, University of California, Berkeley, Berkeley, United States.<br /><searchLink fieldCode="AU" term="%22Cox+JS%22">Cox JS</searchLink>; Department of Molecular and Cell Biology, Division of Immunology and Pathogenesis, University of California, Berkeley, Berkeley, United States.<br /><searchLink fieldCode="AU" term="%22Barton+GM%22">Barton GM</searchLink>; Department of Molecular and Cell Biology, Division of Immunology and Pathogenesis, University of California, Berkeley, Berkeley, United States.<br /><searchLink fieldCode="AU" term="%22Stanley+SA%22">Stanley SA</searchLink>; Department of Molecular and Cell Biology, Division of Immunology and Pathogenesis, University of California, Berkeley, Berkeley, United States.; School of Public Health, Division of Infectious Diseases and Vaccinology, University of California, Berkeley, Berkeley, United States.
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