A multimodal adaptive optical microscope for in vivo imaging from molecules to organisms.

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Title: A multimodal adaptive optical microscope for in vivo imaging from molecules to organisms.
Authors: Fu TM; Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA, USA.; Department of Electrical and Computer Engineering, and Omenn Darling Bioengineering Institute, Princeton University, Princeton, NJ, USA., Liu G; Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, CA, USA., Milkie DE; Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA, USA., Ruan X; Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, CA, USA., Görlitz F; Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, CA, USA., Shi Y; Lampe Joint Department of Biomedical Engineering, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA.; Department of Physics and Astronomy, Western University, London, Ontario, Canada., Ferro V; Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, CA, USA., Divekar NS; Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, CA, USA., Wang W; Department of Chemistry, University of Illinois Chicago, Chicago, IL, USA., York HM; Monash Biomedicine Discovery Institute, Faculty of Medicine, Nursing and Health Sciences, Monash University, Clayton/Melbourne, Victoria, Australia., Kilic V; Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA, USA.; Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, CA, USA., Mueller M; Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA, USA.; Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, CA, USA., Liang Y; Department of Diagnostic Radiology and Nuclear Medicine, University of Maryland School of Medicine, Baltimore, MD, USA., Daugird TA; Department of Pharmacology, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA.; Department of Genetics, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA., Gacha-Garay MJ; Department of Biochemistry and Cell Biology, Stony Brook University, Stony Brook, NY, USA., Larkin KA; Department of Biochemistry and Cell Biology, Stony Brook University, Stony Brook, NY, USA., Adikes RC; Department of Biochemistry and Cell Biology, Stony Brook University, Stony Brook, NY, USA.; Department of Biology, Siena College, Loudonville, NY, USA., Harrison N; Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, CA, USA., Shirazinejad C; Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, CA, USA.; Biophysics Graduate Group, University of California, Berkeley, Berkeley, CA, USA., Williams S; Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, CA, USA., Nourse JL; Department of Physiology and Biophysics, Sue and Bill Gross Stem Cell Research Center, University of California, Irvine, Irvine, CA, USA., Sheu SH; Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA, USA.; Chan Zuckerberg Imaging Institute, Redwood City, CA, USA., Gao L; Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA, USA., Li T; Department of Biology, Howard Hughes Medical Institute, Stanford University, Stanford, CA, USA.; Liangzhu Laboratory, MOE Frontier Science Center for Brain Science and Brain-machine Integration, State Key Laboratory of Brain-machine Intelligence, Zhejiang University, Hangzhou, China., Mondal C; Department of Medicine, Division of Hematology and Oncology, The Tisch Cancer Institute, Icahn School of Medicine at Mount Sinai, New York, NY, USA., Achour K; Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, CA, USA., Hercule W; Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, CA, USA., Stabley DR; Neuroimaging Laboratory, Department of Developmental Neurobiology, St. Jude Children's Research Hospital, Memphis, TN, USA., Emmerich K; Wilmer Eye Institute and the Department of Ophthalmology, Johns Hopkins University School of Medicine, Baltimore, MD, USA.; McKusick-Nathans Institute and the Department of Genetic Medicine, Johns Hopkins University School of Medicine, Baltimore, MD, USA., Dong P; Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA, USA.; Institute of Biomedical and Health Engineering, Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, Shenzhen, China., Drubin DG; Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, CA, USA., Liu ZJ; Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA, USA., Mumm JS; Wilmer Eye Institute and the Department of Ophthalmology, Johns Hopkins University School of Medicine, Baltimore, MD, USA.; McKusick-Nathans Institute and the Department of Genetic Medicine, Johns Hopkins University School of Medicine, Baltimore, MD, USA., Koyama M; Department of Cell and Systems Biology, University of Toronto, Scarborough, Ontario, Canada., Killilea AN; Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, CA, USA., Bravo-Cordero JJ; Department of Medicine, Division of Hematology and Oncology, The Tisch Cancer Institute, Icahn School of Medicine at Mount Sinai, New York, NY, USA., Keene CD; University of Washington BioRepository and Integrated Neuropathology (BRaIN) laboratory, Harborview Medical Center, Seattle, WA, USA., Luo L; Department of Biology, Howard Hughes Medical Institute, Stanford University, Stanford, CA, USA., Kirchhausen T; Program in Cellular and Molecular Medicine, Boston Children's Hospital, Boston, MA, USA.; Department of Pediatrics, Harvard Medical School, Boston, MA, USA.; Department of Cell Biology, Harvard Medical School, Boston, MA, USA., Pathak MM; Department of Physiology and Biophysics, Sue and Bill Gross Stem Cell Research Center, University of California, Irvine, Irvine, CA, USA.; Department of Biomedical Engineering, and Center for Complex Systems Biology, University of California, Irvine, Irvine, CA, USA., Arumugam S; Monash Biomedicine Discovery Institute, Faculty of Medicine, Nursing and Health Sciences, Monash University, Clayton/Melbourne, Victoria, Australia.; European Molecular Biology Laboratory Australia, Monash University, Clayton/Melbourne, Victoria, Australia., Nuñez JK; Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, CA, USA., Gao R; Department of Chemistry, University of Illinois Chicago, Chicago, IL, USA.; Department of Biological Sciences, University of Illinois Chicago, Chicago, IL, USA., Matus DQ; Department of Biochemistry and Cell Biology, Stony Brook University, Stony Brook, NY, USA.; Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, CA, USA., Martin BL; Department of Biochemistry and Cell Biology, Stony Brook University, Stony Brook, NY, USA., Swinburne IA; Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, CA, USA., Betzig E; Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA, USA. betzige@janelia.hhmi.org.; Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, CA, USA. betzige@janelia.hhmi.org.; Department of Physics, Howard Hughes Medical Institute, Helen Wills Neuroscience Institute, University of California, Berkeley, Berkeley, CA, USA. betzige@janelia.hhmi.org., Legant WR; Lampe Joint Department of Biomedical Engineering, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA. legantw@email.unc.edu.; Department of Pharmacology, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA. legantw@email.unc.edu., Upadhyayula S; Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, CA, USA. sup@berkeley.edu.; Molecular Biophysics and Integrated Bioimaging Division, Lawrence Berkeley National Laboratory, Berkeley, CA, USA. sup@berkeley.edu.; Chan Zuckerberg Biohub, San Francisco, CA, USA. sup@berkeley.edu.
Source: Nature methods [Nat Methods] 2026 Jun; Vol. 23 (6), pp. 1184-1195. Date of Electronic Publication: 2026 May 22.
Publication Type: Journal Article
Journal Info: Publisher: Nature Pub. Group Country of Publication: United States NLM ID: 101215604 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1548-7105 (Electronic) Linking ISSN: 15487091 NLM ISO Abbreviation: Nat Methods Subsets: MEDLINE
Database: MEDLINE Ultimate
Description
ISSN:1548-7105
DOI:10.1038/s41592-026-03066-1