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Modeling 3D Glioblastoma: A review of spheroid culture techniques and standardization challenges.

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Title: Modeling 3D Glioblastoma: A review of spheroid culture techniques and standardization challenges.
Authors: Castro LM; RISE-Health, Department of Medical Sciences, Faculty of Health Sciences, University of Beira Interior, Av. Infante D. Henrique, 6250-506 Covilhã, Portugal., Paiva L; RISE-Health, Department of Medical Sciences, Faculty of Health Sciences, University of Beira Interior, Av. Infante D. Henrique, 6250-506 Covilhã, Portugal., Lobo I; RISE-Health, Department of Medical Sciences, Faculty of Health Sciences, University of Beira Interior, Av. Infante D. Henrique, 6250-506 Covilhã, Portugal., Neves AR; RISE-Health, Department of Medical Sciences, Faculty of Health Sciences, University of Beira Interior, Av. Infante D. Henrique, 6250-506 Covilhã, Portugal., Albuquerque T; RISE-Health, Department of Medical Sciences, Faculty of Health Sciences, University of Beira Interior, Av. Infante D. Henrique, 6250-506 Covilhã, Portugal., Sousa Â; RISE-Health, Department of Medical Sciences, Faculty of Health Sciences, University of Beira Interior, Av. Infante D. Henrique, 6250-506 Covilhã, Portugal., Costa D; RISE-Health, Department of Medical Sciences, Faculty of Health Sciences, University of Beira Interior, Av. Infante D. Henrique, 6250-506 Covilhã, Portugal. Electronic address: dcosta@fcsaude.ubi.pt.
Source: Methods (San Diego, Calif.) [Methods] 2026 Sep; Vol. 253, pp. 129-141. Date of Electronic Publication: 2026 May 22.
Publication Type: Journal Article; Review
Journal Info: Publisher: Academic Press Country of Publication: United States NLM ID: 9426302 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1095-9130 (Electronic) Linking ISSN: 10462023 NLM ISO Abbreviation: Methods Subsets: MEDLINE
Database: MEDLINE Ultimate
Description
ISSN:1095-9130
DOI:10.1016/j.ymeth.2026.05.013