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Modeling 3D Glioblastoma: A review of spheroid culture techniques and standardization challenges.

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Title: Modeling 3D Glioblastoma: A review of spheroid culture techniques and standardization challenges.
Authors: Castro LM; RISE-Health, Department of Medical Sciences, Faculty of Health Sciences, University of Beira Interior, Av. Infante D. Henrique, 6250-506 Covilhã, Portugal., Paiva L; RISE-Health, Department of Medical Sciences, Faculty of Health Sciences, University of Beira Interior, Av. Infante D. Henrique, 6250-506 Covilhã, Portugal., Lobo I; RISE-Health, Department of Medical Sciences, Faculty of Health Sciences, University of Beira Interior, Av. Infante D. Henrique, 6250-506 Covilhã, Portugal., Neves AR; RISE-Health, Department of Medical Sciences, Faculty of Health Sciences, University of Beira Interior, Av. Infante D. Henrique, 6250-506 Covilhã, Portugal., Albuquerque T; RISE-Health, Department of Medical Sciences, Faculty of Health Sciences, University of Beira Interior, Av. Infante D. Henrique, 6250-506 Covilhã, Portugal., Sousa Â; RISE-Health, Department of Medical Sciences, Faculty of Health Sciences, University of Beira Interior, Av. Infante D. Henrique, 6250-506 Covilhã, Portugal., Costa D; RISE-Health, Department of Medical Sciences, Faculty of Health Sciences, University of Beira Interior, Av. Infante D. Henrique, 6250-506 Covilhã, Portugal. Electronic address: dcosta@fcsaude.ubi.pt.
Source: Methods (San Diego, Calif.) [Methods] 2026 Sep; Vol. 253, pp. 129-141. Date of Electronic Publication: 2026 May 22.
Publication Type: Journal Article; Review
Journal Info: Publisher: Academic Press Country of Publication: United States NLM ID: 9426302 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1095-9130 (Electronic) Linking ISSN: 10462023 NLM ISO Abbreviation: Methods Subsets: MEDLINE
Database: MEDLINE Ultimate
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  Data: <searchLink fieldCode="AU" term="%22Castro+LM%22">Castro LM</searchLink>; RISE-Health, Department of Medical Sciences, Faculty of Health Sciences, University of Beira Interior, Av. Infante D. Henrique, 6250-506 Covilhã, Portugal.<br /><searchLink fieldCode="AU" term="%22Paiva+L%22">Paiva L</searchLink>; RISE-Health, Department of Medical Sciences, Faculty of Health Sciences, University of Beira Interior, Av. Infante D. Henrique, 6250-506 Covilhã, Portugal.<br /><searchLink fieldCode="AU" term="%22Lobo+I%22">Lobo I</searchLink>; RISE-Health, Department of Medical Sciences, Faculty of Health Sciences, University of Beira Interior, Av. Infante D. Henrique, 6250-506 Covilhã, Portugal.<br /><searchLink fieldCode="AU" term="%22Neves+AR%22">Neves AR</searchLink>; RISE-Health, Department of Medical Sciences, Faculty of Health Sciences, University of Beira Interior, Av. Infante D. Henrique, 6250-506 Covilhã, Portugal.<br /><searchLink fieldCode="AU" term="%22Albuquerque+T%22">Albuquerque T</searchLink>; RISE-Health, Department of Medical Sciences, Faculty of Health Sciences, University of Beira Interior, Av. Infante D. Henrique, 6250-506 Covilhã, Portugal.<br /><searchLink fieldCode="AU" term="%22Sousa+Â%22">Sousa Â</searchLink>; RISE-Health, Department of Medical Sciences, Faculty of Health Sciences, University of Beira Interior, Av. Infante D. Henrique, 6250-506 Covilhã, Portugal.<br /><searchLink fieldCode="AU" term="%22Costa+D%22">Costa D</searchLink>; RISE-Health, Department of Medical Sciences, Faculty of Health Sciences, University of Beira Interior, Av. Infante D. Henrique, 6250-506 Covilhã, Portugal. Electronic address: dcosta@fcsaude.ubi.pt.
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  Data: <i>Publisher: </i><searchLink fieldCode="PB" term="%22Academic+Press%22">Academic Press </searchLink><i>Country of Publication: </i>United States <i>NLM ID: </i>9426302 <i>Publication Model: </i>Print-Electronic <i>Cited Medium: </i>Internet <i>ISSN: </i>1095-9130 (Electronic) <i>Linking ISSN: </i><searchLink fieldCode="IS" term="%2210462023%22">10462023 </searchLink><i>NLM ISO Abbreviation: </i>Methods <i>Subsets: </i>MEDLINE
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        Value: 10.1016/j.ymeth.2026.05.013
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        Text: English
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        StartPage: 129
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      – TitleFull: Modeling 3D Glioblastoma: A review of spheroid culture techniques and standardization challenges.
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              M: 09
              Text: 2026 Sep
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