Rapid Affinity Characterization of Cell-Free Expressed Nanobodies Directly in Lysate with Biolayer Interferometry via Antigen-Immobilized Biosensors.
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| Title: | Rapid Affinity Characterization of Cell-Free Expressed Nanobodies Directly in Lysate with Biolayer Interferometry via Antigen-Immobilized Biosensors. |
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| Authors: | Lange B; Department of Chemical and Biological Engineering, Iowa State University, Ames, Iowa 50011, United States., Godin R; Department of Chemical and Biological Engineering, Iowa State University, Ames, Iowa 50011, United States., Schnicker NJ; Department of Chemical and Biological Engineering, Iowa State University, Ames, Iowa 50011, United States.; Protein Structure, Analysis, and Design Core, University of Iowa, Iowa City, Iowa 52242, United States.; Department of Molecular Physiology and Biophysics, University of Iowa, Iowa City, Iowa 52242, United States., Wadas TJ; Department of Radiology, University of Iowa, Iowa City, Iowa 52242, United States., Reuel NF; Department of Chemical and Biological Engineering, Iowa State University, Ames, Iowa 50011, United States. |
| Source: | ACS synthetic biology [ACS Synth Biol] 2026 Jul 17; Vol. 15 (7), pp. 2925-2935. Date of Electronic Publication: 2026 Jun 16. |
| Publication Type: | Journal Article |
| Journal Info: | Publisher: American Chemical Society Country of Publication: United States NLM ID: 101575075 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 2161-5063 (Electronic) Linking ISSN: 21615063 NLM ISO Abbreviation: ACS Synth Biol Subsets: MEDLINE |
| Database: | MEDLINE Ultimate |
| ISSN: | 2161-5063 |
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| DOI: | 10.1021/acssynbio.6c00062 |
