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Rapid Affinity Characterization of Cell-Free Expressed Nanobodies Directly in Lysate with Biolayer Interferometry via Antigen-Immobilized Biosensors.

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Title: Rapid Affinity Characterization of Cell-Free Expressed Nanobodies Directly in Lysate with Biolayer Interferometry via Antigen-Immobilized Biosensors.
Authors: Lange B; Department of Chemical and Biological Engineering, Iowa State University, Ames, Iowa 50011, United States., Godin R; Department of Chemical and Biological Engineering, Iowa State University, Ames, Iowa 50011, United States., Schnicker NJ; Department of Chemical and Biological Engineering, Iowa State University, Ames, Iowa 50011, United States.; Protein Structure, Analysis, and Design Core, University of Iowa, Iowa City, Iowa 52242, United States.; Department of Molecular Physiology and Biophysics, University of Iowa, Iowa City, Iowa 52242, United States., Wadas TJ; Department of Radiology, University of Iowa, Iowa City, Iowa 52242, United States., Reuel NF; Department of Chemical and Biological Engineering, Iowa State University, Ames, Iowa 50011, United States.
Source: ACS synthetic biology [ACS Synth Biol] 2026 Jul 17; Vol. 15 (7), pp. 2925-2935. Date of Electronic Publication: 2026 Jun 16.
Publication Type: Journal Article
Journal Info: Publisher: American Chemical Society Country of Publication: United States NLM ID: 101575075 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 2161-5063 (Electronic) Linking ISSN: 21615063 NLM ISO Abbreviation: ACS Synth Biol Subsets: MEDLINE
Database: MEDLINE Ultimate
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PubType: Academic Journal
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  Data: <searchLink fieldCode="AU" term="%22Lange+B%22">Lange B</searchLink>; Department of Chemical and Biological Engineering, Iowa State University, Ames, Iowa 50011, United States.<br /><searchLink fieldCode="AU" term="%22Godin+R%22">Godin R</searchLink>; Department of Chemical and Biological Engineering, Iowa State University, Ames, Iowa 50011, United States.<br /><searchLink fieldCode="AU" term="%22Schnicker+NJ%22">Schnicker NJ</searchLink>; Department of Chemical and Biological Engineering, Iowa State University, Ames, Iowa 50011, United States.; Protein Structure, Analysis, and Design Core, University of Iowa, Iowa City, Iowa 52242, United States.; Department of Molecular Physiology and Biophysics, University of Iowa, Iowa City, Iowa 52242, United States.<br /><searchLink fieldCode="AU" term="%22Wadas+TJ%22">Wadas TJ</searchLink>; Department of Radiology, University of Iowa, Iowa City, Iowa 52242, United States.<br /><searchLink fieldCode="AU" term="%22Reuel+NF%22">Reuel NF</searchLink>; Department of Chemical and Biological Engineering, Iowa State University, Ames, Iowa 50011, United States.
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  Data: <searchLink fieldCode="JN" term="%22101575075%22">ACS synthetic biology</searchLink> [ACS Synth Biol] 2026 Jul 17; Vol. 15 (7), pp. 2925-2935. <i>Date of Electronic Publication: </i>2026 Jun 16.
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        Value: 10.1021/acssynbio.6c00062
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      – Code: eng
        Text: English
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      – TitleFull: Rapid Affinity Characterization of Cell-Free Expressed Nanobodies Directly in Lysate with Biolayer Interferometry via Antigen-Immobilized Biosensors.
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              Text: 2026 Jul 17
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