Active Pocket Engineering of d-Tagatose 4-Epimerase for Improved Catalytic Performance and Efficient Cascade Synthesis of d-Tagatose from d-Glucose.

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Title: Active Pocket Engineering of d-Tagatose 4-Epimerase for Improved Catalytic Performance and Efficient Cascade Synthesis of d-Tagatose from d-Glucose.
Authors: Zhang YS; State Key Laboratory of Green Chemical Synthesis and Conversion, Zhejiang University of Technology, Hangzhou 310014, P.R. China.; The National and Local Joint Engineering Research Center for Biomanufacturing of Chiral Chemicals, Zhejiang University of Technology, Hangzhou 310014., P.R. China.; Zhejiang Key Laboratory of Bioorganic Synthesis, College of Biotechnology and Bioengineering Zhejiang University of Technology, Hangzhou 310014, P.R. China., Li ZQ; State Key Laboratory of Green Chemical Synthesis and Conversion, Zhejiang University of Technology, Hangzhou 310014, P.R. China.; The National and Local Joint Engineering Research Center for Biomanufacturing of Chiral Chemicals, Zhejiang University of Technology, Hangzhou 310014., P.R. China.; Zhejiang Key Laboratory of Bioorganic Synthesis, College of Biotechnology and Bioengineering Zhejiang University of Technology, Hangzhou 310014, P.R. China., Chen JH; State Key Laboratory of Green Chemical Synthesis and Conversion, Zhejiang University of Technology, Hangzhou 310014, P.R. China.; The National and Local Joint Engineering Research Center for Biomanufacturing of Chiral Chemicals, Zhejiang University of Technology, Hangzhou 310014., P.R. China.; Zhejiang Key Laboratory of Bioorganic Synthesis, College of Biotechnology and Bioengineering Zhejiang University of Technology, Hangzhou 310014, P.R. China., Dong SA; State Key Laboratory of Green Chemical Synthesis and Conversion, Zhejiang University of Technology, Hangzhou 310014, P.R. China.; The National and Local Joint Engineering Research Center for Biomanufacturing of Chiral Chemicals, Zhejiang University of Technology, Hangzhou 310014., P.R. China.; Zhejiang Key Laboratory of Bioorganic Synthesis, College of Biotechnology and Bioengineering Zhejiang University of Technology, Hangzhou 310014, P.R. China., Cai X; State Key Laboratory of Green Chemical Synthesis and Conversion, Zhejiang University of Technology, Hangzhou 310014, P.R. China.; The National and Local Joint Engineering Research Center for Biomanufacturing of Chiral Chemicals, Zhejiang University of Technology, Hangzhou 310014., P.R. China.; Zhejiang Key Laboratory of Bioorganic Synthesis, College of Biotechnology and Bioengineering Zhejiang University of Technology, Hangzhou 310014, P.R. China., Jin LQ; State Key Laboratory of Green Chemical Synthesis and Conversion, Zhejiang University of Technology, Hangzhou 310014, P.R. China.; The National and Local Joint Engineering Research Center for Biomanufacturing of Chiral Chemicals, Zhejiang University of Technology, Hangzhou 310014., P.R. China.; Zhejiang Key Laboratory of Bioorganic Synthesis, College of Biotechnology and Bioengineering Zhejiang University of Technology, Hangzhou 310014, P.R. China., Liu ZQ; State Key Laboratory of Green Chemical Synthesis and Conversion, Zhejiang University of Technology, Hangzhou 310014, P.R. China.; The National and Local Joint Engineering Research Center for Biomanufacturing of Chiral Chemicals, Zhejiang University of Technology, Hangzhou 310014., P.R. China.; Zhejiang Key Laboratory of Bioorganic Synthesis, College of Biotechnology and Bioengineering Zhejiang University of Technology, Hangzhou 310014, P.R. China., Zheng YG; State Key Laboratory of Green Chemical Synthesis and Conversion, Zhejiang University of Technology, Hangzhou 310014, P.R. China.; The National and Local Joint Engineering Research Center for Biomanufacturing of Chiral Chemicals, Zhejiang University of Technology, Hangzhou 310014., P.R. China.; Zhejiang Key Laboratory of Bioorganic Synthesis, College of Biotechnology and Bioengineering Zhejiang University of Technology, Hangzhou 310014, P.R. China.
Source: Journal of agricultural and food chemistry [J Agric Food Chem] 2026 Jul 15; Vol. 74 (27), pp. 21412-21422. Date of Electronic Publication: 2026 Jul 04.
Publication Type: Journal Article
Journal Info: Publisher: American Chemical Society Country of Publication: United States NLM ID: 0374755 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1520-5118 (Electronic) Linking ISSN: 00218561 NLM ISO Abbreviation: J Agric Food Chem Subsets: MEDLINE
Database: MEDLINE Ultimate
Description
ISSN:1520-5118
DOI:10.1021/acs.jafc.6c08765