Bibliographic Details
| Title: |
Lack of efficacy of ‘naked’ small interfering RNA applied directly to rat brain. |
| Authors: |
Isacson, R., Kull, B., Salmi, P., Wahlestedt, C. |
| Source: |
Acta Physiologica Scandinavica. Oct2003, Vol. 179 Issue 2, p173. 5p. |
| Subjects: |
RNA, Brain, Dopamine receptors |
| Abstract: |
Abstract The intrastriatal infusions of ‘naked’ small interfering RNA (siRNA) targeted to dopamine D[sub 1] receptors (1.0–10.0 nmol over 3 days) did not reduce dopamine D[sub 1] receptor messenger RNA levels or receptor protein, assessed by [[sup 125] I] SCH 23982 binding in intact rats. This was in contrast to results in vitro where a 76% reduction in dopamine D[sub 1] receptor ligand binding could be observed. Conclusion: The results suggest that synthetic siRNA, when applied directly to rat brain, is not capable of inducing RNA interference. [ABSTRACT FROM AUTHOR] |
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| Database: |
Psychology and Behavioral Sciences Collection |
