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Lack of efficacy of ‘naked’ small interfering RNA applied directly to rat brain.

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Title: Lack of efficacy of ‘naked’ small interfering RNA applied directly to rat brain.
Authors: Isacson, R., Kull, B., Salmi, P., Wahlestedt, C.
Source: Acta Physiologica Scandinavica. Oct2003, Vol. 179 Issue 2, p173. 5p.
Subjects: RNA, Brain, Dopamine receptors
Abstract: Abstract The intrastriatal infusions of ‘naked’ small interfering RNA (siRNA) targeted to dopamine D[sub 1] receptors (1.0–10.0 nmol over 3 days) did not reduce dopamine D[sub 1] receptor messenger RNA levels or receptor protein, assessed by [[sup 125] I] SCH 23982 binding in intact rats. This was in contrast to results in vitro where a 76% reduction in dopamine D[sub 1] receptor ligand binding could be observed. Conclusion: The results suggest that synthetic siRNA, when applied directly to rat brain, is not capable of inducing RNA interference. [ABSTRACT FROM AUTHOR]
Copyright of Acta Physiologica Scandinavica is the property of Wiley-Blackwell and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
Database: Psychology and Behavioral Sciences Collection
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  Data: Lack of efficacy of ‘naked’ small interfering RNA applied directly to rat brain.
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  Data: <searchLink fieldCode="AR" term="%22Isacson%2C+R%2E%22">Isacson, R.</searchLink><br /><searchLink fieldCode="AR" term="%22Kull%2C+B%2E%22">Kull, B.</searchLink><br /><searchLink fieldCode="AR" term="%22Salmi%2C+P%2E%22">Salmi, P.</searchLink><br /><searchLink fieldCode="AR" term="%22Wahlestedt%2C+C%2E%22">Wahlestedt, C.</searchLink>
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  Data: <searchLink fieldCode="JN" term="%22Acta+Physiologica+Scandinavica%22">Acta Physiologica Scandinavica</searchLink>. Oct2003, Vol. 179 Issue 2, p173. 5p.
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  Data: <searchLink fieldCode="DE" term="%22RNA%22">RNA</searchLink><br /><searchLink fieldCode="DE" term="%22Brain%22">Brain</searchLink><br /><searchLink fieldCode="DE" term="%22Dopamine+receptors%22">Dopamine receptors</searchLink>
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  Label: Abstract
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  Data: Abstract The intrastriatal infusions of ‘naked’ small interfering RNA (siRNA) targeted to dopamine D[sub 1] receptors (1.0–10.0 nmol over 3 days) did not reduce dopamine D[sub 1] receptor messenger RNA levels or receptor protein, assessed by [[sup 125] I] SCH 23982 binding in intact rats. This was in contrast to results in vitro where a 76% reduction in dopamine D[sub 1] receptor ligand binding could be observed. Conclusion: The results suggest that synthetic siRNA, when applied directly to rat brain, is not capable of inducing RNA interference. [ABSTRACT FROM AUTHOR]
– Name: AbstractSuppliedCopyright
  Label:
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  Data: <i>Copyright of Acta Physiologica Scandinavica is the property of Wiley-Blackwell and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.)
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        Value: 10.1046/j.1365-201X.2003.01188.x
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