Growth dynamics of gut microbiota in health and disease inferred from single metagenomic samples.

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Bibliographic Details
Title:	Growth dynamics of gut microbiota in health and disease inferred from single metagenomic samples.
Authors:	Korem, Tal, Zeevi, David, Suez, Jotham, Weinberger, Adina, Avnit-Sagi, Tali, Pompan-Lotan, Maya, Matot, Elad, Jona, Ghil, Harmelin, Alon, Cohen, Nadav, Sirota-Madi, Alexandra, Thaiss, Christoph A., Pevsner-Fischer, Meirav, Sorek, Rotem, Xavier, Ramnik J., Elinav, Eran, Segal, Eran
Source:	Science (pre-March 2025). 9/4/2015, Vol. 349 Issue 6252, p1101-1106. 6p.
Subjects:	Microbiota, Metagenomics, Bacterial growth, Bacterial reproduction, Gut microbiota, Inflammatory bowel diseases, Type 2 diabetes
Abstract:	Metagenomic sequencing increased our understanding of the role of the microbiome in health and disease, yet it only provides a snapshot of a highly dynamic ecosystem. Here, we show that the pattern of metagenomic sequencing read coverage for different microbial genomes contains a single trough and a single peak, the latter coinciding with the bacterial origin of replication. Furthermore, the ratio of sequencing coverage between the peak and trough provides a quantitative measure of a species' growth rate. We demonstrate this in vitro and in vivo, under different growth conditions, and in complex bacterial communities. For several bacterial species, peak-to-trough coverage ratios, but not relative abundances, correlated with the manifestation of inflammatory bowel disease and type II diabetes. [ABSTRACT FROM AUTHOR]
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Database:	Psychology and Behavioral Sciences Collection

Description
Abstract:	Metagenomic sequencing increased our understanding of the role of the microbiome in health and disease, yet it only provides a snapshot of a highly dynamic ecosystem. Here, we show that the pattern of metagenomic sequencing read coverage for different microbial genomes contains a single trough and a single peak, the latter coinciding with the bacterial origin of replication. Furthermore, the ratio of sequencing coverage between the peak and trough provides a quantitative measure of a species' growth rate. We demonstrate this in vitro and in vivo, under different growth conditions, and in complex bacterial communities. For several bacterial species, peak-to-trough coverage ratios, but not relative abundances, correlated with the manifestation of inflammatory bowel disease and type II diabetes. [ABSTRACT FROM AUTHOR]
ISSN:	00368075
DOI:	10.1126/science.aac4812