Bibliographic Details
| Title: |
Term-seq reveals abundant ribo-regulation of antibiotics resistance in bacteria. |
| Authors: |
Dar, Daniel, Shamir, Maya, Mellin, J. R., Koutero, Mikael, Stern-Ginossar, Noam, Cossart, Pascale, Sorek, Rotem |
| Source: |
Science (pre-March 2025). 4/8/2016, Vol. 352 Issue 6282, paad9822-1-aad9822-12. 12p. |
| Subjects: |
Riboswitches, Antibiotics, Drug resistance in bacteria, Gene expression in bacteria, Nucleotide sequence |
| Abstract: |
Riboswitches and attenuators are cis-regulatory RNA elements,most of which control bacterial gene expression via metabolite-mediated, premature transcription termination.We developed an unbiased experimental approach for genome-wide discovery of such ribo-regulators in bacteria.We also devised an experimental platform that quantitatively measures the in vivo activity of all such regulators in parallel and enables rapid screening for ribo-regulators that respond to metabolites of choice. Using this approach, we detected numerous antibioticresponsive ribo-regulators that control antibiotic resistance genes in pathogens and in the human microbiome. Studying one such regulator in Listeria monocytogenes revealed an attenuation mechanism mediated by antibiotic-stalled ribosomes. Our results expose broad roles for conditional termination in regulating antibiotic resistance and provide a tool for discovering riboswitches and attenuators that respond to previously unknown ligands. [ABSTRACT FROM AUTHOR] |
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| Database: |
Psychology and Behavioral Sciences Collection |
