Term-seq reveals abundant ribo-regulation of antibiotics resistance in bacteria.
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| Title: | Term-seq reveals abundant ribo-regulation of antibiotics resistance in bacteria. |
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| Authors: | Dar, Daniel, Shamir, Maya, Mellin, J. R., Koutero, Mikael, Stern-Ginossar, Noam, Cossart, Pascale, Sorek, Rotem |
| Source: | Science (pre-March 2025). 4/8/2016, Vol. 352 Issue 6282, paad9822-1-aad9822-12. 12p. |
| Subjects: | Riboswitches, Antibiotics, Drug resistance in bacteria, Gene expression in bacteria, Nucleotide sequence |
| Abstract: | Riboswitches and attenuators are cis-regulatory RNA elements,most of which control bacterial gene expression via metabolite-mediated, premature transcription termination.We developed an unbiased experimental approach for genome-wide discovery of such ribo-regulators in bacteria.We also devised an experimental platform that quantitatively measures the in vivo activity of all such regulators in parallel and enables rapid screening for ribo-regulators that respond to metabolites of choice. Using this approach, we detected numerous antibioticresponsive ribo-regulators that control antibiotic resistance genes in pathogens and in the human microbiome. Studying one such regulator in Listeria monocytogenes revealed an attenuation mechanism mediated by antibiotic-stalled ribosomes. Our results expose broad roles for conditional termination in regulating antibiotic resistance and provide a tool for discovering riboswitches and attenuators that respond to previously unknown ligands. [ABSTRACT FROM AUTHOR] |
| Copyright of Science (pre-March 2025) is the property of American Association for the Advancement of Science and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) | |
| Database: | Psychology and Behavioral Sciences Collection |
| FullText | Links: – Type: pdflink Text: Availability: 0 |
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| Header | DbId: pbh DbLabel: Psychology and Behavioral Sciences Collection An: 114447899 AccessLevel: 6 PubType: Academic Journal PubTypeId: academicJournal PreciseRelevancyScore: 0 |
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| Items | – Name: Title Label: Title Group: Ti Data: Term-seq reveals abundant ribo-regulation of antibiotics resistance in bacteria. – Name: Author Label: Authors Group: Au Data: <searchLink fieldCode="AR" term="%22Dar%2C+Daniel%22">Dar, Daniel</searchLink><br /><searchLink fieldCode="AR" term="%22Shamir%2C+Maya%22">Shamir, Maya</searchLink><br /><searchLink fieldCode="AR" term="%22Mellin%2C+J%2E+R%2E%22">Mellin, J. R.</searchLink><br /><searchLink fieldCode="AR" term="%22Koutero%2C+Mikael%22">Koutero, Mikael</searchLink><br /><searchLink fieldCode="AR" term="%22Stern-Ginossar%2C+Noam%22">Stern-Ginossar, Noam</searchLink><br /><searchLink fieldCode="AR" term="%22Cossart%2C+Pascale%22">Cossart, Pascale</searchLink><br /><searchLink fieldCode="AR" term="%22Sorek%2C+Rotem%22">Sorek, Rotem</searchLink> – Name: TitleSource Label: Source Group: Src Data: <searchLink fieldCode="JN" term="%22Science+%28pre-March+2025%29%22">Science (pre-March 2025)</searchLink>. 4/8/2016, Vol. 352 Issue 6282, paad9822-1-aad9822-12. 12p. – Name: Subject Label: Subjects Group: Su Data: <searchLink fieldCode="DE" term="%22Riboswitches%22">Riboswitches</searchLink><br /><searchLink fieldCode="DE" term="%22Antibiotics%22">Antibiotics</searchLink><br /><searchLink fieldCode="DE" term="%22Drug+resistance+in+bacteria%22">Drug resistance in bacteria</searchLink><br /><searchLink fieldCode="DE" term="%22Gene+expression+in+bacteria%22">Gene expression in bacteria</searchLink><br /><searchLink fieldCode="DE" term="%22Nucleotide+sequence%22">Nucleotide sequence</searchLink> – Name: Abstract Label: Abstract Group: Ab Data: Riboswitches and attenuators are cis-regulatory RNA elements,most of which control bacterial gene expression via metabolite-mediated, premature transcription termination.We developed an unbiased experimental approach for genome-wide discovery of such ribo-regulators in bacteria.We also devised an experimental platform that quantitatively measures the in vivo activity of all such regulators in parallel and enables rapid screening for ribo-regulators that respond to metabolites of choice. Using this approach, we detected numerous antibioticresponsive ribo-regulators that control antibiotic resistance genes in pathogens and in the human microbiome. Studying one such regulator in Listeria monocytogenes revealed an attenuation mechanism mediated by antibiotic-stalled ribosomes. Our results expose broad roles for conditional termination in regulating antibiotic resistance and provide a tool for discovering riboswitches and attenuators that respond to previously unknown ligands. [ABSTRACT FROM AUTHOR] – Name: AbstractSuppliedCopyright Label: Group: Ab Data: <i>Copyright of Science (pre-March 2025) is the property of American Association for the Advancement of Science and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.) |
| PLink | https://search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=pbh&AN=114447899 |
| RecordInfo | BibRecord: BibEntity: Identifiers: – Type: doi Value: 10.1126/science.aad9822 Languages: – Code: eng Text: English PhysicalDescription: Pagination: PageCount: 12 StartPage: aad9822-1 Subjects: – SubjectFull: Riboswitches Type: general – SubjectFull: Antibiotics Type: general – SubjectFull: Drug resistance in bacteria Type: general – SubjectFull: Gene expression in bacteria Type: general – SubjectFull: Nucleotide sequence Type: general Titles: – TitleFull: Term-seq reveals abundant ribo-regulation of antibiotics resistance in bacteria. Type: main BibRelationships: HasContributorRelationships: – PersonEntity: Name: NameFull: Dar, Daniel – PersonEntity: Name: NameFull: Shamir, Maya – PersonEntity: Name: NameFull: Mellin, J. R. – PersonEntity: Name: NameFull: Koutero, Mikael – PersonEntity: Name: NameFull: Stern-Ginossar, Noam – PersonEntity: Name: NameFull: Cossart, Pascale – PersonEntity: Name: NameFull: Sorek, Rotem IsPartOfRelationships: – BibEntity: Dates: – D: 08 M: 04 Text: 4/8/2016 Type: published Y: 2016 Identifiers: – Type: issn-print Value: 00368075 Numbering: – Type: volume Value: 352 – Type: issue Value: 6282 Titles: – TitleFull: Science (pre-March 2025) Type: main |
| ResultId | 1 |
