Increased spatiotemporal resolution reveals highly dynamic dense tubular matrices in the peripheral ER.

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Bibliographic Details
Title:	Increased spatiotemporal resolution reveals highly dynamic dense tubular matrices in the peripheral ER.
Authors:	Nixon-Abell, Jonathon (AUTHOR), Obara, Christopher J. (AUTHOR), Weigel, Aubrey V. (AUTHOR), Dong Li (AUTHOR), Legant, Wesley R. (AUTHOR), Xu, C. Shan (AUTHOR), Pasolli, H. Amalia (AUTHOR), Harvey, Kirsten (AUTHOR), Hess, Harald F. (AUTHOR), Betzig, Eric (AUTHOR), Blackstone, Craig (AUTHOR), Lippincott-Schwartz, Jennifer (AUTHOR)
Source:	Science (pre-March 2025). 10/28/2016, Vol. 354 Issue 6311, paaf3928-1-aaf3928-12. 12p. 4 Color Photographs, 1 Black and White Photograph, 3 Graphs.
Subjects:	Endoplasmic reticulum, High resolution imaging, Morphology, Genetic overexpression, Confounding variables, Cell anatomy
Abstract:	The endoplasmic reticulum (ER) is an expansive, membrane-enclosed organelle that plays crucial roles in numerous cellular functions. We used emerging superresolution imaging technologies to clarify the morphology and dynamics of the peripheral ER, which contacts and modulates most other intracellular organelles. Peripheral components of the ER have classically been described as comprising both tubules and flat sheets. We show that this system consists almost exclusively of tubules at varying densities, including structures that we term ER matrices. Conventional optical imaging technologies had led to misidentification of these structures as sheets because of the dense clustering of tubular junctions and a previously uncharacterized rapid form of ER motion. The existence of ER matrices explains previous confounding evidence that had indicated the occurrence of ER “sheet” proliferation after overexpression of tubular junction–forming proteins. [ABSTRACT FROM AUTHOR]
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Database:	Psychology and Behavioral Sciences Collection

Description
Abstract:	The endoplasmic reticulum (ER) is an expansive, membrane-enclosed organelle that plays crucial roles in numerous cellular functions. We used emerging superresolution imaging technologies to clarify the morphology and dynamics of the peripheral ER, which contacts and modulates most other intracellular organelles. Peripheral components of the ER have classically been described as comprising both tubules and flat sheets. We show that this system consists almost exclusively of tubules at varying densities, including structures that we term ER matrices. Conventional optical imaging technologies had led to misidentification of these structures as sheets because of the dense clustering of tubular junctions and a previously uncharacterized rapid form of ER motion. The existence of ER matrices explains previous confounding evidence that had indicated the occurrence of ER “sheet” proliferation after overexpression of tubular junction–forming proteins. [ABSTRACT FROM AUTHOR]
ISSN:	00368075
DOI:	10.1126/science.aaf3928