Metabolome modification and underlying biomarker of noise-induced hearing loss Guinea pig cochlear fluid.
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| Title: | Metabolome modification and underlying biomarker of noise-induced hearing loss Guinea pig cochlear fluid. |
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| Authors: | Qi, Guowei (AUTHOR), Tang, Jinge (AUTHOR), Qin, Handai (AUTHOR), Han, Runnan (AUTHOR), Jiang, Qingqing (AUTHOR), Yu, Ning (AUTHOR), Yang, Shiming (AUTHOR), Han, Dongyi (AUTHOR) |
| Source: | Acta Oto-Laryngologica. Feb2025, Vol. 145 Issue 2, p101-114. 14p. |
| Subjects: | Guinea pigs, Noise-induced deafness, Research funding, Liquid chromatography-mass spectrometry, Phosphorylation, Phospholipids, Gas chromatography, Energy metabolism, Animal experimentation, Mass spectrometry, Metabolism, Metabolomics, Cochlea, Biomarkers |
| Abstract (English): | Background: Noise-induced hearing loss (NIHL) is a kind of acquired sensorineural hearing loss and has shown an increasing incidence in recent years. Hence, elucidating the exact pathophysiological mechanisms and proposing effective treatment and prevention methods become the top priority. Though a great number of researches have been carried out on NIHL, few of them were focused on metabolites. Aims/Objectives: To reveal the metabolomic changes in cochlear fluid after noise injury and search for underlying inner ear biomarkers of NIHL. Material and methods: In this study, cochlea fluid extracted from guinea pigs after impulse noise exposure were subjected to GC-MS and LC-MS untargeted metabolomics analysis. Results: After impulse noise exposure, 62 significantly changed metabolites in guinea pig cochlea fluid were screened out and deoxyribose 1-phosphate was selected as the key metabolite and underlying biomarker for NIHL. KEGG pathway analysis showed that oxidative phosphorylation, glycerophospholipid metabolism, pyrimidine metabolism and pentose phosphate pathway were significantly changed at all observed time points after noise. Conclusions and significance: This study effectively promoted the application of metabolomics in hearing research. The pathophysiology process of NIHL in the inner ear was closely connected with oxidative phosphorylation, glycerophospholipid metabolism, pyrimidine metabolism and pentose phosphate pathway and deoxyribose 1-phosphate could be the biomarker for NIHL. [ABSTRACT FROM AUTHOR] |
| Abstract (Chinese): | 噪声性听力损失(NIHL)是一种获得性神经性听力损失, 近年来发病率不断上升。因此, 阐明其确切的病理生理机制并提出有效的治疗和预防措施成为当务之急。 尽管对NIHL进行了大量研究, 但其中很少有研究关注代谢物。 揭示噪声损伤后耳蜗液代谢组学的变化, 寻找NIHL的潜在内耳生物标志物。 本研究对暴露于脉冲噪声后的豚鼠提取的耳蜗液进行GC-MS和LC-MS非靶向代谢组学分析。 将豚鼠暴露于脉冲噪声后, 筛选出豚鼠耳蜗液中62种显著变化的代谢物, 并选择脱氧核糖1-磷酸作为NIHL的关键代谢物和潜在生物标志物。 KEGG通路分析显示, 噪声暴露后各观察时间点的氧化磷酸化、甘油磷脂代谢、嘧啶代谢及磷酸戊糖途径均发生明显改变。 本研究有效提倡了代谢组学在听力研究中的应用。内耳NIHL的病理生理过程与氧化磷酸化、甘油磷脂代谢、嘧啶代谢及磷酸戊糖途径密切相关；脱氧核糖1-磷酸可作为NIHL的生物标志物。 [ABSTRACT FROM AUTHOR] |
| Copyright of Acta Oto-Laryngologica is the property of Taylor & Francis Ltd and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) | |
| Database: | Psychology and Behavioral Sciences Collection |
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| Abstract: | Background: Noise-induced hearing loss (NIHL) is a kind of acquired sensorineural hearing loss and has shown an increasing incidence in recent years. Hence, elucidating the exact pathophysiological mechanisms and proposing effective treatment and prevention methods become the top priority. Though a great number of researches have been carried out on NIHL, few of them were focused on metabolites. Aims/Objectives: To reveal the metabolomic changes in cochlear fluid after noise injury and search for underlying inner ear biomarkers of NIHL. Material and methods: In this study, cochlea fluid extracted from guinea pigs after impulse noise exposure were subjected to GC-MS and LC-MS untargeted metabolomics analysis. Results: After impulse noise exposure, 62 significantly changed metabolites in guinea pig cochlea fluid were screened out and deoxyribose 1-phosphate was selected as the key metabolite and underlying biomarker for NIHL. KEGG pathway analysis showed that oxidative phosphorylation, glycerophospholipid metabolism, pyrimidine metabolism and pentose phosphate pathway were significantly changed at all observed time points after noise. Conclusions and significance: This study effectively promoted the application of metabolomics in hearing research. The pathophysiology process of NIHL in the inner ear was closely connected with oxidative phosphorylation, glycerophospholipid metabolism, pyrimidine metabolism and pentose phosphate pathway and deoxyribose 1-phosphate could be the biomarker for NIHL. [ABSTRACT FROM AUTHOR] |
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| ISSN: | 00016489 |
| DOI: | 10.1080/00016489.2024.2445738 |
