Effect of basic-fibroblast growth factor on tympanic membrane perforation in a mouse model of blast injury.
Saved in:
| Title: | Effect of basic-fibroblast growth factor on tympanic membrane perforation in a mouse model of blast injury. |
|---|---|
| Authors: | Sato, Motohiro (AUTHOR), Mizutari, Kunio (AUTHOR), Kurioka, Takaomi (AUTHOR), Inuzuka, Yoshiaki (AUTHOR), Yasutake, Shingo (AUTHOR), Araki, Koji (AUTHOR), Satoh, Yasushi (AUTHOR), Shiotani, Akihiro (AUTHOR) |
| Source: | Acta Oto-Laryngologica. May2025, Vol. 145 Issue 5, p382-389. 8p. |
| Subjects: | Tympanic membrane perforation, Biological models, Otitis media, Treatment effectiveness, Mice, Chronic diseases, Regeneration (Biology), Fibroblast growth factors, Animal experimentation, Inner ear, Histological techniques, Blast injuries, Comparative studies, Hearing levels, Hair cells, Disease complications |
| Abstract (English): | Background: Tympanic membrane perforation (TMP) caused by blast injury result in poor TMP closure and pathological regeneration of the tympanic membrane (TM). TM regeneration therapy using basic fibroblast growth factor (bFGF) has demonstrated good outcomes for chronic otitis media. Aims/Objectives: To investigate the efficacy of bFGF treatment in the management of blast-induced TMP. Material and methods: Male CBA/J mice (10–12-week-old) were divided into the bFGF-treatment and no-treatment groups. Blast-tube systems were used to induce TMP. Auditory evaluation, TM observation, histological evaluation of the regenerated TM, and histological evaluation of the inner ear were conducted. Results: Auditory assessment revealed no difference between the groups, with persistent auditory threshold elevation being observed even 1 month post-injury. Similarly, histological evaluation of the inner ear hair cells revealed no significant differences between the groups; no hair cell death was evident. However, the regenerated TM in the bFGF-treated group was significantly thicker than that in the untreated group. Conclusions and significance: A good TMP closure rate was observed in the present study; however, bFGF treatment exacerbated thickening of the regenerated TM. Future studies must study the therapeutic effects of bFGF treatment using various animal models and timing and concentration of bFGF treatment. [ABSTRACT FROM AUTHOR] |
| Abstract (Chinese): | 爆炸伤引起的鼓膜穿孔 (TMP) 导致 TMP 闭合不良和鼓膜 (TM) 病理再生。使用碱性成纤维细胞生长因子 (bFGF) 的 TM 再生疗法对慢性中耳炎具有良好的效果。 研究 bFGF 治疗dui 治疗爆炸引起的 TMP 的疗效。 雄性 CBA/J 小鼠(10-12 周龄)分为 bFGF 治疗组和无治疗组。使用爆-管系统诱发TMP。进行了听觉评估、TM 观察、再生 TM 的组织学评估和内耳的组织学评估。 听觉评估显示各组之间没有差异, 即使在受伤后 1 个月仍观察到持续的听觉阈值升高。同样, 内耳毛细胞的组织学评估显示各组之间没有显著差异；无毛细胞死亡。然而, bFGF治疗组的再生TM明显比无治疗组的厚。 本研究观察到良好的TMP闭合率；然而, bFGF治疗加剧了再生TM的增厚。未来的研究必须使用各种动物模型以及bFGF治疗的时间和浓度来研究bFGF治疗的治疗效果。 [ABSTRACT FROM AUTHOR] |
| Copyright of Acta Oto-Laryngologica is the property of Taylor & Francis Ltd and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) | |
| Database: | Psychology and Behavioral Sciences Collection |
|
Full text is not displayed to guests.
Login for full access.
|
|
| Abstract: | Background: Tympanic membrane perforation (TMP) caused by blast injury result in poor TMP closure and pathological regeneration of the tympanic membrane (TM). TM regeneration therapy using basic fibroblast growth factor (bFGF) has demonstrated good outcomes for chronic otitis media. Aims/Objectives: To investigate the efficacy of bFGF treatment in the management of blast-induced TMP. Material and methods: Male CBA/J mice (10–12-week-old) were divided into the bFGF-treatment and no-treatment groups. Blast-tube systems were used to induce TMP. Auditory evaluation, TM observation, histological evaluation of the regenerated TM, and histological evaluation of the inner ear were conducted. Results: Auditory assessment revealed no difference between the groups, with persistent auditory threshold elevation being observed even 1 month post-injury. Similarly, histological evaluation of the inner ear hair cells revealed no significant differences between the groups; no hair cell death was evident. However, the regenerated TM in the bFGF-treated group was significantly thicker than that in the untreated group. Conclusions and significance: A good TMP closure rate was observed in the present study; however, bFGF treatment exacerbated thickening of the regenerated TM. Future studies must study the therapeutic effects of bFGF treatment using various animal models and timing and concentration of bFGF treatment. [ABSTRACT FROM AUTHOR] |
|---|---|
| ISSN: | 00016489 |
| DOI: | 10.1080/00016489.2025.2470971 |
