Experimental study on the application of irreversible electroporation/nanoknife on tonsil tissues in a rabbit model.
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| Title: | Experimental study on the application of irreversible electroporation/nanoknife on tonsil tissues in a rabbit model. |
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| Authors: | Tao, Xinggang (AUTHOR), Chen, Jiayan (AUTHOR), Li, Zhuo (AUTHOR), Sun, Yihui (AUTHOR), Shan, Chunyu (AUTHOR), Li, Yingfeng (AUTHOR), Li, Qian (AUTHOR), Hao, Quanqin (AUTHOR), Li, Ying (AUTHOR), Yang, Xizhi (AUTHOR), Li, Keyong (AUTHOR) |
| Source: | Acta Oto-Laryngologica. Jul2025, Vol. 145 Issue 7, p654-660. 7p. |
| Subjects: | Biological models, Leukocytes, Ablation techniques, Research funding, Heart injuries, Apoptosis, Electroporation, Radio frequency therapy, Guided tissue regeneration, Experimental design, Creatine kinase, Animal experimentation, Tonsils, Catheter ablation, Comparative studies, Lymphoid tissue, Inflammation, Rabbits, Cells, C-reactive protein |
| Abstract (English): | Background: Irreversible electroporation (IRE)/nanoknife is a nonthermal technique for ablating solid tumors. Objectives: To investigate the application of IRE for ablating tonsil tissues in a rabbit model. Material and Methods: Forty five rabbits were divided into three groups IRE, radiofrequency ablation (RFA) and control group. The IRE group received IRE in one or both tonsils, while the RFA group received IRE in one tonsil and RFA in the other. The control group had no operation. Rabbits were euthanized at days 1, 3, 7, 14, and 28 post-IRE ablation or RFA. White blood cell (WBC) counts, C-reactive protein (CRP), and creatine kinase (CK) levels were monitored and tonsil tissues were histologically examined. Results: IRE ablation induced cell apoptosis in tonsil tissues, which was most pronounced at 72 h post-treatment. At 14- and 28-days post-IRE ablation, lymphoid follicles regenerated in the tonsil tissues. The RFA group showed more severe injury and less tissue regeneration. Transient increases in WBC, CRP, and CK levels indicated inflammation and cardiac injury induced by IRE. Conclusions and Significance: IRE is a potential modality for ablating tonsil tissues, which avoids thermal injury and preserving blood vessels. More robust studies with other animal models are required for definitive evidence. [ABSTRACT FROM AUTHOR] |
| Abstract (Chinese): | 不可逆电穿孔 (IRE)/纳米刀是消融实体肿瘤的一种非热技术。 探讨 IRE 在消融兔扁桃体组织中的应用。 45 只家兔分为 IRE 组、射频消融 (RFA) 组和对照组。IRE 组, 在一侧或两侧扁桃体接受 IRE 治疗；RFA 组, 在一侧扁桃体接受 IRE 治疗, 在另一侧扁桃体接受 RFA 治疗。对照组不接受手术。在 IRE 消融或 RFA 治疗后第 1、3、7、14 和 28 天对家兔实施安乐死。监测白细胞 (WBC) 计数、C 反应蛋白 (CRP) 和肌酸激酶 (CK) 水平, 并对扁桃体组织进行组织学检查。 IRE消融诱发了扁桃体组织细胞凋亡, 在治疗后72小时最为明显。IRE消融后的14天和28天, 扁桃体组织中淋巴滤泡再生。RFA组损伤更为严重, 组织再生较少。WBC、CRP和CK水平的短暂性升高提示IRE诱导了炎症和心脏损伤。 IRE是消融扁桃体组织的一种潜在方法, 可避免热损伤并保护血管。需要对其他动物模型进行更深入的研究以获得确凿证据。 [ABSTRACT FROM AUTHOR] |
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| Database: | Psychology and Behavioral Sciences Collection |
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| Abstract: | Background: Irreversible electroporation (IRE)/nanoknife is a nonthermal technique for ablating solid tumors. Objectives: To investigate the application of IRE for ablating tonsil tissues in a rabbit model. Material and Methods: Forty five rabbits were divided into three groups IRE, radiofrequency ablation (RFA) and control group. The IRE group received IRE in one or both tonsils, while the RFA group received IRE in one tonsil and RFA in the other. The control group had no operation. Rabbits were euthanized at days 1, 3, 7, 14, and 28 post-IRE ablation or RFA. White blood cell (WBC) counts, C-reactive protein (CRP), and creatine kinase (CK) levels were monitored and tonsil tissues were histologically examined. Results: IRE ablation induced cell apoptosis in tonsil tissues, which was most pronounced at 72 h post-treatment. At 14- and 28-days post-IRE ablation, lymphoid follicles regenerated in the tonsil tissues. The RFA group showed more severe injury and less tissue regeneration. Transient increases in WBC, CRP, and CK levels indicated inflammation and cardiac injury induced by IRE. Conclusions and Significance: IRE is a potential modality for ablating tonsil tissues, which avoids thermal injury and preserving blood vessels. More robust studies with other animal models are required for definitive evidence. [ABSTRACT FROM AUTHOR] |
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| ISSN: | 00016489 |
| DOI: | 10.1080/00016489.2025.2491652 |
