Regulation of angiogenesis by a non-canonical Wnt-Flt1 pathway in myeloid cells.

Saved in:

Bibliographic Details
Title:	Regulation of angiogenesis by a non-canonical Wnt-Flt1 pathway in myeloid cells.
Authors:	Stefater III, James A., Lewkowich, Ian, Rao, Sujata, Mariggi, Giovanni, Carpenter, April C., Burr, Adam R., Fan, Jieqing, Ajima, Rieko, Molkentin, Jeffery D., Williams, Bart O., Wills-Karp, Marsha, Pollard, Jeffrey W., Yamaguchi, Terry, Ferrara, Napoleone, Gerhardt, Holger, Lang, Richard A.
Source:	Nature. 6/23/2011, Vol. 474 Issue 7352, p511-515. 5p. 4 Graphs.
Subjects:	Neovascularization, Vascular endothelial growth factors, Vascular endothelium, Retinal ganglion cells, Wnt proteins
Abstract:	Myeloid cells are a feature of most tissues. Here we show that during development, retinal myeloid cells (RMCs) produce Wnt ligands to regulate blood vessel branching. In the mouse retina, where angiogenesis occurs postnatally, somatic deletion in RMCs of the Wnt ligand transporter Wntless results in increased angiogenesis in the deeper layers. We also show that mutation of Wnt5a and Wnt11 results in increased angiogenesis and that these ligands elicit RMC responses via a non-canonical Wnt pathway. Using cultured myeloid-like cells and RMC somatic deletion of Flt1, we show that an effector of Wnt-dependent suppression of angiogenesis by RMCs is Flt1, a naturally occurring inhibitor of vascular endothelial growth factor (VEGF). These findings indicate that resident myeloid cells can use a non-canonical, Wnt-Flt1 pathway to suppress angiogenic branching. [ABSTRACT FROM AUTHOR]
	Copyright of Nature is the property of Springer Nature and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
Database:	Psychology and Behavioral Sciences Collection

Description
Abstract:	Myeloid cells are a feature of most tissues. Here we show that during development, retinal myeloid cells (RMCs) produce Wnt ligands to regulate blood vessel branching. In the mouse retina, where angiogenesis occurs postnatally, somatic deletion in RMCs of the Wnt ligand transporter Wntless results in increased angiogenesis in the deeper layers. We also show that mutation of Wnt5a and Wnt11 results in increased angiogenesis and that these ligands elicit RMC responses via a non-canonical Wnt pathway. Using cultured myeloid-like cells and RMC somatic deletion of Flt1, we show that an effector of Wnt-dependent suppression of angiogenesis by RMCs is Flt1, a naturally occurring inhibitor of vascular endothelial growth factor (VEGF). These findings indicate that resident myeloid cells can use a non-canonical, Wnt-Flt1 pathway to suppress angiogenic branching. [ABSTRACT FROM AUTHOR]
ISSN:	00280836
DOI:	10.1038/nature10085