Decreased phorbol ester binding in the parahippocampal gyrus from subjects with schizophrenia is not associated with changes in protein kinase C.

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Title:	Decreased phorbol ester binding in the parahippocampal gyrus from subjects with schizophrenia is not associated with changes in protein kinase C.
Authors:	Scarr, E., Pavey, G., Robinson, P.J., Opeskin, K., Copolov, D.L., Dean, B.
Source:	Molecular Psychiatry. 2002, Vol. 7 Issue 7, p683-688. 6p.
Subjects:	Phorbol esters, Schizophrenia, Protein kinase C, Hippocampus (Brain)
Abstract:	Combining in situ radioligand binding with autoradiography, we previously identified a reduction of [³H]phorbol 12,13-dibutyrate binding in the parahippocampal gyrus from schizophrenic subjects. To determine whether these changes were due to decreases in the level of protein kinase C, we measured [³H]phorbol 12,13-dibutyrate binding, levels of the protein kinase C isoforms α, δ, ε, γ, η, and θ as well as protein kinase C activity in crude particulate membranes from parahippocampal gyri of 15 schizophrenic and 15 control subjects. There was a significant decrease in the density (mean ± SEM: 6.56 ± 0.73 pmol mg[sup -1] vs 9.68 ± 1.22 pmol mg[sup -1]; P < 0.05) and affinity (mean K[sub D] ± SEM: 4.64 ± 0.34 nM vs 2.95 ± 0.35 nM; P < 0.005) of [³H]phorbol 12,13-dibutyrate binding in homogenates from schizophrenic subjects. There were no significant changes in levels of the protein kinase C isoforms which are known to bind phorbol esters or in the activity of protein kinase C in membranes from schizophrenic subjects. These results suggest that there are changes in molecules capable of binding [³H]phorbol 12,13-dibutyrate, other than protein kinase C, in the parahippocampal gyrus from subjects with schizophrenia. [ABSTRACT FROM AUTHOR]
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Database:	Psychology and Behavioral Sciences Collection

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Abstract:	Combining in situ radioligand binding with autoradiography, we previously identified a reduction of [³H]phorbol 12,13-dibutyrate binding in the parahippocampal gyrus from schizophrenic subjects. To determine whether these changes were due to decreases in the level of protein kinase C, we measured [³H]phorbol 12,13-dibutyrate binding, levels of the protein kinase C isoforms α, δ, ε, γ, η, and θ as well as protein kinase C activity in crude particulate membranes from parahippocampal gyri of 15 schizophrenic and 15 control subjects. There was a significant decrease in the density (mean ± SEM: 6.56 ± 0.73 pmol mg[sup -1] vs 9.68 ± 1.22 pmol mg[sup -1]; P < 0.05) and affinity (mean K[sub D] ± SEM: 4.64 ± 0.34 nM vs 2.95 ± 0.35 nM; P < 0.005) of [³H]phorbol 12,13-dibutyrate binding in homogenates from schizophrenic subjects. There were no significant changes in levels of the protein kinase C isoforms which are known to bind phorbol esters or in the activity of protein kinase C in membranes from schizophrenic subjects. These results suggest that there are changes in molecules capable of binding [³H]phorbol 12,13-dibutyrate, other than protein kinase C, in the parahippocampal gyrus from subjects with schizophrenia. [ABSTRACT FROM AUTHOR]
ISSN:	13594184
DOI:	10.1038/sj.mp.4001065