Decreased phorbol ester binding in the parahippocampal gyrus from subjects with schizophrenia is not associated with changes in protein kinase C.
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| Title: | Decreased phorbol ester binding in the parahippocampal gyrus from subjects with schizophrenia is not associated with changes in protein kinase C. |
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| Authors: | Scarr, E., Pavey, G., Robinson, P.J., Opeskin, K., Copolov, D.L., Dean, B. |
| Source: | Molecular Psychiatry. 2002, Vol. 7 Issue 7, p683-688. 6p. |
| Subjects: | Phorbol esters, Schizophrenia, Protein kinase C, Hippocampus (Brain) |
| Abstract: | Combining in situ radioligand binding with autoradiography, we previously identified a reduction of [³H]phorbol 12,13-dibutyrate binding in the parahippocampal gyrus from schizophrenic subjects. To determine whether these changes were due to decreases in the level of protein kinase C, we measured [³H]phorbol 12,13-dibutyrate binding, levels of the protein kinase C isoforms α, δ, ε, γ, η, and θ as well as protein kinase C activity in crude particulate membranes from parahippocampal gyri of 15 schizophrenic and 15 control subjects. There was a significant decrease in the density (mean ± SEM: 6.56 ± 0.73 pmol mg[sup -1] vs 9.68 ± 1.22 pmol mg[sup -1]; P < 0.05) and affinity (mean K[sub D] ± SEM: 4.64 ± 0.34 nM vs 2.95 ± 0.35 nM; P < 0.005) of [³H]phorbol 12,13-dibutyrate binding in homogenates from schizophrenic subjects. There were no significant changes in levels of the protein kinase C isoforms which are known to bind phorbol esters or in the activity of protein kinase C in membranes from schizophrenic subjects. These results suggest that there are changes in molecules capable of binding [³H]phorbol 12,13-dibutyrate, other than protein kinase C, in the parahippocampal gyrus from subjects with schizophrenia. [ABSTRACT FROM AUTHOR] |
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| Database: | Psychology and Behavioral Sciences Collection |
| FullText | Links: – Type: pdflink Text: Availability: 0 |
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| Header | DbId: pbh DbLabel: Psychology and Behavioral Sciences Collection An: 8650087 AccessLevel: 6 PubType: Academic Journal PubTypeId: academicJournal PreciseRelevancyScore: 0 |
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| Items | – Name: Title Label: Title Group: Ti Data: Decreased phorbol ester binding in the parahippocampal gyrus from subjects with schizophrenia is not associated with changes in protein kinase C. – Name: Author Label: Authors Group: Au Data: <searchLink fieldCode="AR" term="%22Scarr%2C+E%2E%22">Scarr, E.</searchLink><br /><searchLink fieldCode="AR" term="%22Pavey%2C+G%2E%22">Pavey, G.</searchLink><br /><searchLink fieldCode="AR" term="%22Robinson%2C+P%2EJ%2E%22">Robinson, P.J.</searchLink><br /><searchLink fieldCode="AR" term="%22Opeskin%2C+K%2E%22">Opeskin, K.</searchLink><br /><searchLink fieldCode="AR" term="%22Copolov%2C+D%2EL%2E%22">Copolov, D.L.</searchLink><br /><searchLink fieldCode="AR" term="%22Dean%2C+B%2E%22">Dean, B.</searchLink> – Name: TitleSource Label: Source Group: Src Data: <searchLink fieldCode="JN" term="%22Molecular+Psychiatry%22">Molecular Psychiatry</searchLink>. 2002, Vol. 7 Issue 7, p683-688. 6p. – Name: Subject Label: Subjects Group: Su Data: <searchLink fieldCode="DE" term="%22Phorbol+esters%22">Phorbol esters</searchLink><br /><searchLink fieldCode="DE" term="%22Schizophrenia%22">Schizophrenia</searchLink><br /><searchLink fieldCode="DE" term="%22Protein+kinase+C%22">Protein kinase C</searchLink><br /><searchLink fieldCode="DE" term="%22Hippocampus+%28Brain%29%22">Hippocampus (Brain)</searchLink> – Name: Abstract Label: Abstract Group: Ab Data: Combining in situ radioligand binding with autoradiography, we previously identified a reduction of [³H]phorbol 12,13-dibutyrate binding in the parahippocampal gyrus from schizophrenic subjects. To determine whether these changes were due to decreases in the level of protein kinase C, we measured [³H]phorbol 12,13-dibutyrate binding, levels of the protein kinase C isoforms α, δ, ε, γ, η, and θ as well as protein kinase C activity in crude particulate membranes from parahippocampal gyri of 15 schizophrenic and 15 control subjects. There was a significant decrease in the density (mean ± SEM: 6.56 ± 0.73 pmol mg[sup -1] vs 9.68 ± 1.22 pmol mg[sup -1]; P < 0.05) and affinity (mean K[sub D] ± SEM: 4.64 ± 0.34 nM vs 2.95 ± 0.35 nM; P < 0.005) of [³H]phorbol 12,13-dibutyrate binding in homogenates from schizophrenic subjects. There were no significant changes in levels of the protein kinase C isoforms which are known to bind phorbol esters or in the activity of protein kinase C in membranes from schizophrenic subjects. These results suggest that there are changes in molecules capable of binding [³H]phorbol 12,13-dibutyrate, other than protein kinase C, in the parahippocampal gyrus from subjects with schizophrenia. [ABSTRACT FROM AUTHOR] – Name: AbstractSuppliedCopyright Label: Group: Ab Data: <i>Copyright of Molecular Psychiatry is the property of Springer Nature and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.) |
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| RecordInfo | BibRecord: BibEntity: Identifiers: – Type: doi Value: 10.1038/sj.mp.4001065 Languages: – Code: eng Text: English PhysicalDescription: Pagination: PageCount: 6 StartPage: 683 Subjects: – SubjectFull: Phorbol esters Type: general – SubjectFull: Schizophrenia Type: general – SubjectFull: Protein kinase C Type: general – SubjectFull: Hippocampus (Brain) Type: general Titles: – TitleFull: Decreased phorbol ester binding in the parahippocampal gyrus from subjects with schizophrenia is not associated with changes in protein kinase C. Type: main BibRelationships: HasContributorRelationships: – PersonEntity: Name: NameFull: Scarr, E. – PersonEntity: Name: NameFull: Pavey, G. – PersonEntity: Name: NameFull: Robinson, P.J. – PersonEntity: Name: NameFull: Opeskin, K. – PersonEntity: Name: NameFull: Copolov, D.L. – PersonEntity: Name: NameFull: Dean, B. IsPartOfRelationships: – BibEntity: Dates: – D: 01 M: 07 Text: 2002 Type: published Y: 2002 Identifiers: – Type: issn-print Value: 13594184 Numbering: – Type: volume Value: 7 – Type: issue Value: 7 Titles: – TitleFull: Molecular Psychiatry Type: main |
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