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Long read sequencing reveals transgene concatemerization and vector sequences integration following AAV-driven electroporation of CRISPR RNP complexes in mouse zygotes.

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Title: Long read sequencing reveals transgene concatemerization and vector sequences integration following AAV-driven electroporation of CRISPR RNP complexes in mouse zygotes.
Authors: Luqman MW; Dementia Research Centre, Macquarie Medical School, Faculty of Medicine, Health and Human Sciences, Macquarie University, Sydney, NSW, Australia.; Khyber Medical University, Institute of Medical Sciences, Kohat, Pakistan., Jenjaroenpun P; Division of Medical Bioinformatics, Research Department, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok, Thailand., Spathos J; Dementia Research Centre, Macquarie Medical School, Faculty of Medicine, Health and Human Sciences, Macquarie University, Sydney, NSW, Australia., Shingte N; FOXG1 Research Foundation, New York, NY, United States., Cummins M; Dementia Research Centre, Macquarie Medical School, Faculty of Medicine, Health and Human Sciences, Macquarie University, Sydney, NSW, Australia.; School of Biotechnology and Biomolecular Sciences, The University of New South Wales, Sydney, NSW, Australia., Nimsamer P; Division of Medical Bioinformatics, Research Department, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok, Thailand., Ittner LM; Dementia Research Centre, Macquarie Medical School, Faculty of Medicine, Health and Human Sciences, Macquarie University, Sydney, NSW, Australia., Wongsurawat T; Division of Medical Bioinformatics, Research Department, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok, Thailand., Delerue F; Dementia Research Centre, Macquarie Medical School, Faculty of Medicine, Health and Human Sciences, Macquarie University, Sydney, NSW, Australia.
Source: Frontiers in genome editing [Front Genome Ed] 2025 Jun 04; Vol. 7, pp. 1582097. Date of Electronic Publication: 2025 Jun 04 (Print Publication: 2025).
Publication Type: Journal Article
Journal Info: Publisher: Frontiers Media S.A Country of Publication: Switzerland NLM ID: 101775540 Publication Model: eCollection Cited Medium: Internet ISSN: 2673-3439 (Electronic) Linking ISSN: 26733439 NLM ISO Abbreviation: Front Genome Ed Subsets: PubMed not MEDLINE
Database: MEDLINE Ultimate
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  Data: Long read sequencing reveals transgene concatemerization and vector sequences integration following AAV-driven electroporation of CRISPR RNP complexes in mouse zygotes.
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  Data: <searchLink fieldCode="AU" term="%22Luqman+MW%22">Luqman MW</searchLink>; Dementia Research Centre, Macquarie Medical School, Faculty of Medicine, Health and Human Sciences, Macquarie University, Sydney, NSW, Australia.; Khyber Medical University, Institute of Medical Sciences, Kohat, Pakistan.<br /><searchLink fieldCode="AU" term="%22Jenjaroenpun+P%22">Jenjaroenpun P</searchLink>; Division of Medical Bioinformatics, Research Department, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok, Thailand.<br /><searchLink fieldCode="AU" term="%22Spathos+J%22">Spathos J</searchLink>; Dementia Research Centre, Macquarie Medical School, Faculty of Medicine, Health and Human Sciences, Macquarie University, Sydney, NSW, Australia.<br /><searchLink fieldCode="AU" term="%22Shingte+N%22">Shingte N</searchLink>; FOXG1 Research Foundation, New York, NY, United States.<br /><searchLink fieldCode="AU" term="%22Cummins+M%22">Cummins M</searchLink>; Dementia Research Centre, Macquarie Medical School, Faculty of Medicine, Health and Human Sciences, Macquarie University, Sydney, NSW, Australia.; School of Biotechnology and Biomolecular Sciences, The University of New South Wales, Sydney, NSW, Australia.<br /><searchLink fieldCode="AU" term="%22Nimsamer+P%22">Nimsamer P</searchLink>; Division of Medical Bioinformatics, Research Department, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok, Thailand.<br /><searchLink fieldCode="AU" term="%22Ittner+LM%22">Ittner LM</searchLink>; Dementia Research Centre, Macquarie Medical School, Faculty of Medicine, Health and Human Sciences, Macquarie University, Sydney, NSW, Australia.<br /><searchLink fieldCode="AU" term="%22Wongsurawat+T%22">Wongsurawat T</searchLink>; Division of Medical Bioinformatics, Research Department, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok, Thailand.<br /><searchLink fieldCode="AU" term="%22Delerue+F%22">Delerue F</searchLink>; Dementia Research Centre, Macquarie Medical School, Faculty of Medicine, Health and Human Sciences, Macquarie University, Sydney, NSW, Australia.
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  Data: <searchLink fieldCode="JN" term="%22101775540%22">Frontiers in genome editing</searchLink> [Front Genome Ed] 2025 Jun 04; Vol. 7, pp. 1582097. <i>Date of Electronic Publication: </i>2025 Jun 04 (<i>Print Publication: </i>2025).
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  Data: <i>Publisher: </i><searchLink fieldCode="PB" term="%22Frontiers+Media+S%2EA%22">Frontiers Media S.A </searchLink><i>Country of Publication: </i>Switzerland <i>NLM ID: </i>101775540 <i>Publication Model: </i>eCollection <i>Cited Medium: </i>Internet <i>ISSN: </i>2673-3439 (Electronic) <i>Linking ISSN: </i><searchLink fieldCode="IS" term="%2226733439%22">26733439 </searchLink><i>NLM ISO Abbreviation: </i>Front Genome Ed <i>Subsets: </i>PubMed not MEDLINE
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        Text: English
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              Text: 2025 Jun 04
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