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Active Pocket Engineering of d-Tagatose 4-Epimerase for Improved Catalytic Performance and Efficient Cascade Synthesis of d-Tagatose from d-Glucose.

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Title: Active Pocket Engineering of d-Tagatose 4-Epimerase for Improved Catalytic Performance and Efficient Cascade Synthesis of d-Tagatose from d-Glucose.
Authors: Zhang YS; State Key Laboratory of Green Chemical Synthesis and Conversion, Zhejiang University of Technology, Hangzhou 310014, P.R. China.; The National and Local Joint Engineering Research Center for Biomanufacturing of Chiral Chemicals, Zhejiang University of Technology, Hangzhou 310014., P.R. China.; Zhejiang Key Laboratory of Bioorganic Synthesis, College of Biotechnology and Bioengineering Zhejiang University of Technology, Hangzhou 310014, P.R. China., Li ZQ; State Key Laboratory of Green Chemical Synthesis and Conversion, Zhejiang University of Technology, Hangzhou 310014, P.R. China.; The National and Local Joint Engineering Research Center for Biomanufacturing of Chiral Chemicals, Zhejiang University of Technology, Hangzhou 310014., P.R. China.; Zhejiang Key Laboratory of Bioorganic Synthesis, College of Biotechnology and Bioengineering Zhejiang University of Technology, Hangzhou 310014, P.R. China., Chen JH; State Key Laboratory of Green Chemical Synthesis and Conversion, Zhejiang University of Technology, Hangzhou 310014, P.R. China.; The National and Local Joint Engineering Research Center for Biomanufacturing of Chiral Chemicals, Zhejiang University of Technology, Hangzhou 310014., P.R. China.; Zhejiang Key Laboratory of Bioorganic Synthesis, College of Biotechnology and Bioengineering Zhejiang University of Technology, Hangzhou 310014, P.R. China., Dong SA; State Key Laboratory of Green Chemical Synthesis and Conversion, Zhejiang University of Technology, Hangzhou 310014, P.R. China.; The National and Local Joint Engineering Research Center for Biomanufacturing of Chiral Chemicals, Zhejiang University of Technology, Hangzhou 310014., P.R. China.; Zhejiang Key Laboratory of Bioorganic Synthesis, College of Biotechnology and Bioengineering Zhejiang University of Technology, Hangzhou 310014, P.R. China., Cai X; State Key Laboratory of Green Chemical Synthesis and Conversion, Zhejiang University of Technology, Hangzhou 310014, P.R. China.; The National and Local Joint Engineering Research Center for Biomanufacturing of Chiral Chemicals, Zhejiang University of Technology, Hangzhou 310014., P.R. China.; Zhejiang Key Laboratory of Bioorganic Synthesis, College of Biotechnology and Bioengineering Zhejiang University of Technology, Hangzhou 310014, P.R. China., Jin LQ; State Key Laboratory of Green Chemical Synthesis and Conversion, Zhejiang University of Technology, Hangzhou 310014, P.R. China.; The National and Local Joint Engineering Research Center for Biomanufacturing of Chiral Chemicals, Zhejiang University of Technology, Hangzhou 310014., P.R. China.; Zhejiang Key Laboratory of Bioorganic Synthesis, College of Biotechnology and Bioengineering Zhejiang University of Technology, Hangzhou 310014, P.R. China., Liu ZQ; State Key Laboratory of Green Chemical Synthesis and Conversion, Zhejiang University of Technology, Hangzhou 310014, P.R. China.; The National and Local Joint Engineering Research Center for Biomanufacturing of Chiral Chemicals, Zhejiang University of Technology, Hangzhou 310014., P.R. China.; Zhejiang Key Laboratory of Bioorganic Synthesis, College of Biotechnology and Bioengineering Zhejiang University of Technology, Hangzhou 310014, P.R. China., Zheng YG; State Key Laboratory of Green Chemical Synthesis and Conversion, Zhejiang University of Technology, Hangzhou 310014, P.R. China.; The National and Local Joint Engineering Research Center for Biomanufacturing of Chiral Chemicals, Zhejiang University of Technology, Hangzhou 310014., P.R. China.; Zhejiang Key Laboratory of Bioorganic Synthesis, College of Biotechnology and Bioengineering Zhejiang University of Technology, Hangzhou 310014, P.R. China.
Source: Journal of agricultural and food chemistry [J Agric Food Chem] 2026 Jul 15; Vol. 74 (27), pp. 21412-21422. Date of Electronic Publication: 2026 Jul 04.
Publication Type: Journal Article
Journal Info: Publisher: American Chemical Society Country of Publication: United States NLM ID: 0374755 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1520-5118 (Electronic) Linking ISSN: 00218561 NLM ISO Abbreviation: J Agric Food Chem Subsets: MEDLINE
Database: MEDLINE Ultimate
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PubType: Academic Journal
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  Data: Active Pocket Engineering of d-Tagatose 4-Epimerase for Improved Catalytic Performance and Efficient Cascade Synthesis of d-Tagatose from d-Glucose.
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  Data: <searchLink fieldCode="AU" term="%22Zhang+YS%22">Zhang YS</searchLink>; State Key Laboratory of Green Chemical Synthesis and Conversion, Zhejiang University of Technology, Hangzhou 310014, P.R. China.; The National and Local Joint Engineering Research Center for Biomanufacturing of Chiral Chemicals, Zhejiang University of Technology, Hangzhou 310014., P.R. China.; Zhejiang Key Laboratory of Bioorganic Synthesis, College of Biotechnology and Bioengineering Zhejiang University of Technology, Hangzhou 310014, P.R. China.<br /><searchLink fieldCode="AU" term="%22Li+ZQ%22">Li ZQ</searchLink>; State Key Laboratory of Green Chemical Synthesis and Conversion, Zhejiang University of Technology, Hangzhou 310014, P.R. China.; The National and Local Joint Engineering Research Center for Biomanufacturing of Chiral Chemicals, Zhejiang University of Technology, Hangzhou 310014., P.R. China.; Zhejiang Key Laboratory of Bioorganic Synthesis, College of Biotechnology and Bioengineering Zhejiang University of Technology, Hangzhou 310014, P.R. China.<br /><searchLink fieldCode="AU" term="%22Chen+JH%22">Chen JH</searchLink>; State Key Laboratory of Green Chemical Synthesis and Conversion, Zhejiang University of Technology, Hangzhou 310014, P.R. China.; The National and Local Joint Engineering Research Center for Biomanufacturing of Chiral Chemicals, Zhejiang University of Technology, Hangzhou 310014., P.R. China.; Zhejiang Key Laboratory of Bioorganic Synthesis, College of Biotechnology and Bioengineering Zhejiang University of Technology, Hangzhou 310014, P.R. China.<br /><searchLink fieldCode="AU" term="%22Dong+SA%22">Dong SA</searchLink>; State Key Laboratory of Green Chemical Synthesis and Conversion, Zhejiang University of Technology, Hangzhou 310014, P.R. China.; The National and Local Joint Engineering Research Center for Biomanufacturing of Chiral Chemicals, Zhejiang University of Technology, Hangzhou 310014., P.R. China.; Zhejiang Key Laboratory of Bioorganic Synthesis, College of Biotechnology and Bioengineering Zhejiang University of Technology, Hangzhou 310014, P.R. China.<br /><searchLink fieldCode="AU" term="%22Cai+X%22">Cai X</searchLink>; State Key Laboratory of Green Chemical Synthesis and Conversion, Zhejiang University of Technology, Hangzhou 310014, P.R. China.; The National and Local Joint Engineering Research Center for Biomanufacturing of Chiral Chemicals, Zhejiang University of Technology, Hangzhou 310014., P.R. China.; Zhejiang Key Laboratory of Bioorganic Synthesis, College of Biotechnology and Bioengineering Zhejiang University of Technology, Hangzhou 310014, P.R. China.<br /><searchLink fieldCode="AU" term="%22Jin+LQ%22">Jin LQ</searchLink>; State Key Laboratory of Green Chemical Synthesis and Conversion, Zhejiang University of Technology, Hangzhou 310014, P.R. China.; The National and Local Joint Engineering Research Center for Biomanufacturing of Chiral Chemicals, Zhejiang University of Technology, Hangzhou 310014., P.R. China.; Zhejiang Key Laboratory of Bioorganic Synthesis, College of Biotechnology and Bioengineering Zhejiang University of Technology, Hangzhou 310014, P.R. China.<br /><searchLink fieldCode="AU" term="%22Liu+ZQ%22">Liu ZQ</searchLink>; State Key Laboratory of Green Chemical Synthesis and Conversion, Zhejiang University of Technology, Hangzhou 310014, P.R. China.; The National and Local Joint Engineering Research Center for Biomanufacturing of Chiral Chemicals, Zhejiang University of Technology, Hangzhou 310014., P.R. China.; Zhejiang Key Laboratory of Bioorganic Synthesis, College of Biotechnology and Bioengineering Zhejiang University of Technology, Hangzhou 310014, P.R. China.<br /><searchLink fieldCode="AU" term="%22Zheng+YG%22">Zheng YG</searchLink>; State Key Laboratory of Green Chemical Synthesis and Conversion, Zhejiang University of Technology, Hangzhou 310014, P.R. China.; The National and Local Joint Engineering Research Center for Biomanufacturing of Chiral Chemicals, Zhejiang University of Technology, Hangzhou 310014., P.R. China.; Zhejiang Key Laboratory of Bioorganic Synthesis, College of Biotechnology and Bioengineering Zhejiang University of Technology, Hangzhou 310014, P.R. China.
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  Data: <searchLink fieldCode="JN" term="%220374755%22">Journal of agricultural and food chemistry</searchLink> [J Agric Food Chem] 2026 Jul 15; Vol. 74 (27), pp. 21412-21422. <i>Date of Electronic Publication: </i>2026 Jul 04.
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  Data: <i>Publisher: </i><searchLink fieldCode="PB" term="%22American+Chemical+Society%22">American Chemical Society </searchLink><i>Country of Publication: </i>United States <i>NLM ID: </i>0374755 <i>Publication Model: </i>Print-Electronic <i>Cited Medium: </i>Internet <i>ISSN: </i>1520-5118 (Electronic) <i>Linking ISSN: </i><searchLink fieldCode="IS" term="%2200218561%22">00218561 </searchLink><i>NLM ISO Abbreviation: </i>J Agric Food Chem <i>Subsets: </i>MEDLINE
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RecordInfo BibRecord:
  BibEntity:
    Identifiers:
      – Type: doi
        Value: 10.1021/acs.jafc.6c08765
    Languages:
      – Code: eng
        Text: English
    PhysicalDescription:
      Pagination:
        StartPage: 21412
    Titles:
      – TitleFull: Active Pocket Engineering of d-Tagatose 4-Epimerase for Improved Catalytic Performance and Efficient Cascade Synthesis of d-Tagatose from d-Glucose.
        Type: main
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      – PersonEntity:
          Name:
            NameFull: Zhang YS
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          Name:
            NameFull: Li ZQ
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            NameFull: Chen JH
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            NameFull: Dong SA
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            NameFull: Cai X
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            NameFull: Jin LQ
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            NameFull: Liu ZQ
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            – D: 15
              M: 07
              Text: 2026 Jul 15
              Type: published
              Y: 2026
          Identifiers:
            – Type: issn-electronic
              Value: 1520-5118
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              Value: 74
            – Type: issue
              Value: 27
          Titles:
            – TitleFull: Journal of agricultural and food chemistry
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